Yao Ziang, Wu Haige, Han Baoqin, Liu Wanshun
College of Biomengineer, Dalian University, Dalian 116622, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2006 Aug;23(4):800-4.
Some different membranes were prepared by Chitosan with the degree of deacetylation (DD) of 63.7%, 73.7%, 83% and 97% respectively. To study the biocompatibility of Chitosan membrane toward corneal stromal cells, the rabbit cells were cultured on the surface of different DD chitosan membranes. The morphological characteristics, the cell-adhesion, the cell proliferation and the activity of LDH in the medium were investigated. The results of experiment shows that the DD of Chitosan has very significant effect on the biocompatibility of Chitosan membrane toward corneal stromal cells. The more DD of Chitosan, the less injury was made to corneal stromal cells by the chitosan membrane, which is favor of the growing and adhesion of corneal stromal cells. The biocompatibility of the membrane made with low DD Chitosan with corneal stromal cells became worse.
分别用脱乙酰度（DD）为63.7%、73.7%、83%和97%的壳聚糖制备了一些不同的膜。为了研究壳聚糖膜对角膜基质细胞的生物相容性，将兔细胞培养在不同DD值的壳聚糖膜表面。研究了其形态特征、细胞黏附、细胞增殖以及培养基中乳酸脱氢酶（LDH）的活性。实验结果表明，壳聚糖的DD值对壳聚糖膜与角膜基质细胞的生物相容性有非常显著的影响。壳聚糖的DD值越高，壳聚糖膜对角膜基质细胞的损伤越小，有利于角膜基质细胞的生长和黏附。低DD值壳聚糖制成的膜与角膜基质细胞的生物相容性变差。
