Gao Xingshuang, Liu Wanshun, Han Baoqin, Wei Xiaojuan
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
Sheng Wu Gong Cheng Xue Bao. 2008 Aug;24(8):1381-6.
To study the possibility of using hydroxypropyl chitosan-based blend membranes as carriers of corneal cells in tissue engineering, we prepared three kinds of blend membranes labeled hydroxypropyl chitosan/chondroitin sulfate, hydroxypropyl chitosan/gelatin/chondroitin sulfate and hydroxypropyl chitosan/oxidized hyaluronic acid/chondroitin sulfate. The transparency, water content and ability of protein adsorption of the blend membranes were measured. To evaluate the cytocompatibility of the blend membranes with corneal epithelial cells, rabbit corneal epithelial cells were cultured on the surface of the carrier membranes. The morphological characteristics, cell adhesion, cell proliferation and the activity of lactate dehydrogenase (LDH) in the media were investigated. Three kinds of blend membranes had good optical transmittance, suitable water content and ability of protein adsorption. The results showed that the less injury was made to corneal epithelial cells by the hydroxypropyl chitosan/gelatin/chondroitin sulfate blend membrane than the others. This kind of membrane was favor of the growth and adhesion of corneal epithelial cells. The hydroxypropyl chitosan/gelatin/chondroitin sulfate blend membrane is a promising carrier of corneal cells and can be used in reconstruction of tissue engineered cornea.
为研究基于羟丙基壳聚糖的共混膜作为组织工程中角膜细胞载体的可能性，我们制备了三种共混膜，分别标记为羟丙基壳聚糖/硫酸软骨素、羟丙基壳聚糖/明胶/硫酸软骨素和羟丙基壳聚糖/氧化透明质酸/硫酸软骨素。测定了共混膜的透明度、含水量和蛋白质吸附能力。为评估共混膜与角膜上皮细胞的细胞相容性，将兔角膜上皮细胞培养在载体膜表面。研究了细胞的形态特征、细胞黏附、细胞增殖以及培养基中乳酸脱氢酶（LDH）的活性。三种共混膜均具有良好的透光率、适宜的含水量和蛋白质吸附能力。结果表明，羟丙基壳聚糖/明胶/硫酸软骨素共混膜对角膜上皮细胞造成的损伤比其他两种共混膜小。这种膜有利于角膜上皮细胞的生长和黏附。羟丙基壳聚糖/明胶/硫酸软骨素共混膜是一种很有前景的角膜细胞载体，可用于组织工程角膜的重建。
