Sarada R, Vidhyavathi R, Usha D, Ravishankar G A
Plant Cell Biotechnology Department, Central Food Technological Research Institute, Mysore 570 020, India.
J Agric Food Chem. 2006 Oct 4;54(20):7585-8. doi: 10.1021/jf060737t.
Haematococcus pluvialis is one of the potent organisms for production of astaxanthin, a high value ketocarotenoid. Astaxanthin is accumulated in thick-walled cyst cells of Haematococcus. The thick cell wall is made up of sporopollenin-like material, algaenan, which hinders solvent extraction of astaxanthin. In the present study, an improved method for extraction of astaxanthin without homogenization of cells is reported. Extractability of astaxanthin from cyst cells was evaluated by treating cells with various solvents and pretreating the cells with organic and mineral acids at 70 degrees C followed by acetone extraction. Hydrochloric acid treatment facilitated 86-94% extractability of astaxanthin. Treatment time, temperature, and concentration of the acid were found to be critical factors for maximum extractability. The treatment did not affect the astaxanthin ester profile and the treated cells can be preserved until further use.
雨生红球藻是生产虾青素（一种高价值的酮类胡萝卜素）的有效生物之一。虾青素积累在雨生红球藻的厚壁囊胞细胞中。厚细胞壁由类孢粉素物质、藻烷组成，这阻碍了虾青素的溶剂提取。在本研究中，报道了一种无需细胞匀浆的虾青素提取改进方法。通过用各种溶剂处理细胞，并在70℃下用有机和无机酸预处理细胞，然后进行丙酮提取，评估了囊胞细胞中虾青素的可提取性。盐酸处理使虾青素的可提取率达到86 - 94%。发现处理时间、温度和酸浓度是实现最大可提取率的关键因素。该处理不影响虾青素酯的分布，且处理后的细胞可保存直至进一步使用。
