Choi Y E, Yun Y-S, Park J M
Department of Chemical Engineering, School of Environmental Science and Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Pohang, Korea 790-784.
Biotechnol Prog. 2002 Nov-Dec;18(6):1170-5. doi: 10.1021/bp025549b.
Factors affecting the astaxanthin production by a unicellular green alga, Haematococcus pluvialis UTEX 16, were evaluated with sequential fractional factorial design. To simulate an actual production mode, a two-stage process was adapted for astaxanthin production: the alga was first cultivated under vegetative growth conditions, and then astaxanthin production was induced by applying various induction methods. A high dose of irradiation was most effective for the production of astaxanthin both in weight (mg/g) and in cellular (pg/cell) contents. A combination of nitrogen deficiency and acetate addition also significantly increased the astaxanthin content of cells on a dry weight basis. Meanwhile, the acetate addition alone increased only the cellular content of astaxanthin. Although the addition of ferrous ion alone had a negative effect on the weight content of astaxanthin, simultaneous addition of ferrous ion and acetate was effective for increasing the cellular content of astaxanthin.
采用序贯分式析因设计评估了影响单细胞绿藻雨生红球藻UTEX 16虾青素产量的因素。为模拟实际生产模式，采用两阶段工艺生产虾青素：首先在营养生长条件下培养藻类，然后通过应用各种诱导方法诱导虾青素的产生。高剂量辐照对虾青素的重量（mg/g）和细胞内（pg/细胞）含量的产生最为有效。缺氮和添加乙酸盐的组合也显著提高了以干重计的细胞虾青素含量。同时，单独添加乙酸盐仅增加了虾青素的细胞内含量。尽管单独添加亚铁离子对虾青素的重量含量有负面影响，但同时添加亚铁离子和乙酸盐对增加虾青素的细胞内含量是有效的。
