Armour J A, Yuan B X, Butler C K
Department of Physiology, Faculty of Medicine, Dalhousie University, Halifax, N.S., Canada.
Peptides. 1990 Jul-Aug;11(4):753-61. doi: 10.1016/0196-9781(90)90191-7.
In order to study the effects of peptides on intrinsic cardiac neurons, substance P, bradykinin, oxytocin, calcitonin gene related peptide, atrial natriuretic peptide and vasoactive intestinal peptide were administered into canine atrial or ventricular ganglionated plexi. When substance P was injected into right atrial or cranial medial ventricular ganglionated plexi heart rate, atrial force and ventricular intramyocardial pressures were augmented. No cardiac changes occurred when similar volumes of saline (i.e., peptide vehicle) were injected into these ganglionated plexi. When bradykinin was injected into atrial or ventricular ganglionated plexi heart rate, atrial force and ventricular force were augmented in approximately 50% and depressor responses were elicited in approximately 50% of these animals. When oxytocin was injected into right atrial ventral ganglionated plexi heart rate and atrial forces were reduced in five of ten dogs studied. No cardiac changes occurred when oxytocin was injected into left atrial or ventricular ganglionated plexi. No responses were elicited when calcitonin gene related peptide, atrial natriuretic peptide or vasoactive intestinal peptide was administered into atrial or ventricular ganglionated plexi. Following acute decentralization of the heart, no significant responses were elicited by repeat administrations of substance P, bradykinin or oxytocin, implying that connectivity with central nervous system neurons was necessary for consistent responses to be elicited. It is concluded that substance P, bradykinin and oxytocin can affect neurons on the heart such that cardiodynamics are modified, these different peptides eliciting different cardiac responses.
为了研究肽对心脏固有神经元的作用，将P物质、缓激肽、催产素、降钙素基因相关肽、心房利钠肽和血管活性肠肽注入犬心房或心室神经节丛。当将P物质注入右心房或颅内侧心室神经节丛时，心率、心房力和心室内膜压力增加。当将等量的生理盐水（即肽载体）注入这些神经节丛时，未出现心脏变化。当将缓激肽注入心房或心室神经节丛时，约50%的动物心率、心房力和心室力增加，约50%的动物出现降压反应。当将催产素注入右心房腹侧神经节丛时，在研究的10只狗中有5只心率和心房力降低。当将催产素注入左心房或心室神经节丛时，未出现心脏变化。当将降钙素基因相关肽、心房利钠肽或血管活性肠肽注入心房或心室神经节丛时，未引发反应。心脏急性去神经支配后，重复给予P物质、缓激肽或催产素未引发明显反应，这意味着与中枢神经系统神经元的连接对于引发一致的反应是必要的。得出的结论是，P物质、缓激肽和催产素可影响心脏上的神经元，从而改变心脏动力学，这些不同的肽引发不同的心脏反应。
