Cutillas Cristina, de Rojas Manuel, Ariza Concepción, Ubeda José Manuel, Guevara Diego
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, Profesor García González s/n., Sevilla, Spain.
Parasitol Res. 2007 Jan;100(2):383-9. doi: 10.1007/s00436-006-0275-z. Epub 2006 Sep 27.
Trichuris suis was isolated from the cecum of two different hosts (Sus scrofa domestica -- swine and Sus scrofa scrofa -- wild boar) and Trichuris vulpis from dogs in Sevilla, Spain. Genomic DNA was isolated and internal transcribed spacers (ITS)1-5.8S-ITS2 segment from the ribosomal DNA (rDNA) was amplified and sequenced using polymerase chain reaction techniques. The sequence of T. suis from both hosts was 1,396 bp in length while that of T. vulpis was 1,044 bp. ITS1 of both populations isolated of T. suis was 661 nucleotides in length, while the ITS2 was 534 nucleotides in length. Furthermore, the ITS1 of T. vulpis was 410 nucleotides in length, while the ITS2 was 433 nucleotides in length. One hundred fifty-four nucleotides were observed along the 5.8S gene of T. suis and T. vulpis. Intraindividual and intraspecific variations were detected in the rDNA of both species. The presence of microsatellites was observed in all the individuals assayed. Sequence analysis of the ITSs and the 5.8S gene has demonstrated no sequence differences between T. suis isolated from both hosts (S. scrofa domestica -- swine and S. scrofa scrofa -- wild boar). Nevertheless, clear differences were detected between the ITS1 and ITS2 of T. suis and T. vulpis. Furthermore, a comparative molecular analysis between both species and the previously published ITS1-5.8S-ITS2 sequence data of Trichuris ovis, Trichuris leporis, Trichuris muris, Trichuris arvicolae, and Trichuris skrjabini was carried out. A common homology zone was detected in the ITS1 sequence of all species of trichurids.
猪鞭虫是从两种不同宿主（家猪——Sus scrofa domestica和野猪——Sus scrofa scrofa）的盲肠中分离出来的，狐鞭虫则是从西班牙塞维利亚的狗体内分离得到的。提取基因组DNA，使用聚合酶链反应技术扩增并测序核糖体DNA（rDNA）的内部转录间隔区（ITS）1 - 5.8S - ITS2片段。来自两种宿主的猪鞭虫序列长度为1396 bp，而狐鞭虫的序列长度为1044 bp。分离得到的两种猪鞭虫群体的ITS1长度均为661个核苷酸，ITS2长度均为534个核苷酸。此外，狐鞭虫的ITS1长度为410个核苷酸，ITS2长度为433个核苷酸。在猪鞭虫和狐鞭虫的5.8S基因中观察到154个核苷酸。在这两个物种的rDNA中检测到个体内和种内变异情况。在所检测的所有个体中均观察到微卫星的存在。对ITS和5.8S基因的序列分析表明，从两种宿主（家猪——Sus scrofa domestica和野猪——Sus scrofa scrofa）分离得到的猪鞭虫之间没有序列差异。然而，在猪鞭虫和狐鞭虫的ITS1和ITS2之间检测到明显差异。此外，还对这两个物种与先前发表的绵羊鞭虫、兔鞭虫、鼠鞭虫、田鼠鞭虫和斯氏鞭虫的ITS1 - 5.8S - ITS2序列数据进行了比较分子分析。在所有毛首线虫物种的ITS1序列中检测到一个共同的同源区域。
