Chiriaeva O G, Efimov A M, Smaragdov M G, Finashin S A, Smirnov A F
Tsitologiia. 1990;32(6):626-32.
Cytochemical and molecular peculiarities of heterochromatic regions of bovine chromosomes have been studied, using specific fluorochrome staining induced decondensation, in situ hybridization, pretreatment of restriction enzymes. The heterochromatin of autosomes demonstrated a strong homogeneity. In chromosome Y two small specific heterochromatic regions were found lacking a long repeated tandem block of nucleotides enriched in GC base pairs and having no tandem block of Bkm repeats (10(4) b.p.). This class repeats are probably interspersed in the bovine genome. A rather seldom character of mammalian karyotypes is the absence of cytochemical heterochromatin in the X chromosome.
利用特定荧光染料染色诱导解聚、原位杂交、限制性内切酶预处理等方法,对牛染色体异染色质区域的细胞化学和分子特性进行了研究。常染色体的异染色质表现出很强的同质性。在Y染色体上发现了两个小的特异性异染色质区域,它们缺乏富含GC碱基对的长重复串联核苷酸块,也没有Bkm重复序列(10⁴ 碱基对)的串联块。这类重复序列可能散布在牛的基因组中。哺乳动物核型中一个相当罕见的特征是X染色体中不存在细胞化学异染色质。