通过半定量逆转录聚合酶链反应证实犬钩口线虫成虫的性别特异性转录本

Confirmation of sex-specific transcripts from Ancylostoma caninum adult worms by semi-quantitative RT-PCR.

作者信息

Miranda R R, Clara e Silva L, Santos H A, Rabelo E M

机构信息

Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.

出版信息

Res Vet Sci. 2007 Apr;82(2):215-7. doi: 10.1016/j.rvsc.2006.07.002. Epub 2006 Sep 26.

DOI:10.1016/j.rvsc.2006.07.002

PMID:17005221

Abstract

In a previous work Ancylostoma caninum sex-specific genes had being identified, however without confirmation, by using an non specific RT-PCR comparing male and female cDNA. In this work more fragments had been identified and a semi-quantitative RT-PCR carried out in order to confirm the sex-specific character of the transcripts obtained. Four fragments were confirmed as sex-specific or as being expressed more in one sex than the other.

摘要

在之前的一项研究中，通过使用非特异性逆转录聚合酶链反应（RT-PCR）比较犬钩虫雄雌cDNA，已鉴定出犬钩虫性别特异性基因，但未经证实。在这项研究中，已鉴定出更多片段，并进行了半定量RT-PCR，以确认所获得转录本的性别特异性特征。四个片段被确认为性别特异性片段，或在某一性别中表达量高于另一性别。