Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos 6627, Campus Pampulha, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil.
Mol Cell Probes. 2009 Dec;23(6):298-303. doi: 10.1016/j.mcp.2009.07.004. Epub 2009 Jul 29.
Various transcripts coding for proteins considered to be central to parasite-host interactions were identified previously as male-enriched in the hookworm Ancylostoma braziliense. Among these genes were an ASP-5-like homologue and a Kunitz-type protease inhibitor. The present study extends this previous work to investigate similar molecules in other hookworms (Ancylostomatidae). Specifically, partial cDNA sequences encoding three different ASP molecules and two different Kunitz-type protease inhibitors were isolated, and the differential transcription between adult male and female worms was compared by conventional and quantitative reverse transcription (RT)-PCR for three species, A. braziliense, Ancylostoma caninum and Ancylostoma ceylanicum. In accordance with previous findings, male-enriched transcription was observed for all molecules explored. Based on this information, it is hypothesized that adult males are responsible for producing proteins essential to the survival of hookworms inside the host and for supporting developmental and reproductive processes in female worms.
先前已鉴定出与寄生虫-宿主相互作用相关的蛋白质的各种转录本，在巴西钩虫Ancylostoma braziliense 中被认为是雄性富集的。这些基因包括一个 ASP-5 样同源物和一个 Kunitz 型蛋白酶抑制剂。本研究扩展了之前的工作，以研究其他钩虫（Ancylostomatidae）中的类似分子。具体来说，通过常规和定量逆转录（RT）-PCR 分离编码三种不同 ASP 分子和两种不同 Kunitz 型蛋白酶抑制剂的部分 cDNA 序列，并比较了三种物种（巴西钩虫 Ancylostoma braziliense、犬钩虫Ancylostoma caninum 和斯里兰卡钩虫Ancylostoma ceylanicum）的雌雄成虫之间的差异转录。与先前的发现一致，所有探索的分子都观察到雄性富集转录。基于这些信息，可以假设雄性成虫负责产生对钩虫在宿主内生存至关重要的蛋白质，并支持雌性成虫的发育和生殖过程。
