Lu Wenfa, Rawlings Norman, Zhao Jing, Wang Heng
College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, PR China.
Anim Reprod Sci. 2007 Jul;100(1-2):186-91. doi: 10.1016/j.anireprosci.2006.08.023. Epub 2006 Aug 26.
A fast and reliable method for bovine sexing has been developed through amplification of the bovine high motility group (HMG) box of the sex-determining region of the Y chromosome gene (SRY). Oligonucleotide primers were designed according to the conserved bovine SRY HMG box sequence motif. In agarose gel electrophoresis, a normal bull showed 1 SRY band, and a normal cow showed no SRY band. After optimization, the PCR procedure for sex determination was applied to 14 embryo biopsies. The biopsied embryos were transferred into 14 recipient cows on the same day (day 7 of the estrus cycle) that the embryos were collected and sex of the calf was confirmed after parturition. Nine calves were born and anatomical sex corresponded to those sex determined by PCR in all cases (100% accuracy). Thus, this study showed for the first time that the present method can be applied in bovine breeding programs to facilitate manipulation of the sex ratio of offspring and also allows a quick diagnosis for the XY-bovine offspring by amplification of the HMG box of the bovine SRY gene.
通过扩增Y染色体基因（SRY）性别决定区域的牛高迁移率族（HMG）框，已开发出一种快速可靠的牛性别鉴定方法。根据保守的牛SRY HMG框序列基序设计了寡核苷酸引物。在琼脂糖凝胶电泳中，正常公牛显示出1条SRY带，正常母牛未显示SRY带。优化后，将性别鉴定的PCR程序应用于14份胚胎活检样本。活检后的胚胎在采集当天（发情周期第7天）被移植到14头受体母牛体内，产犊后确认犊牛性别。产下9头犊牛，所有情况下解剖学性别均与PCR鉴定的性别一致（准确率100%）。因此，本研究首次表明，该方法可应用于牛的育种计划，以促进对后代性别比例的控制，并且通过扩增牛SRY基因的HMG框，还可对XY型牛后代进行快速诊断。
