Zeng Y T, Zhang M L, Chen M J, Zhou X D, Huang Y, Ren Z R, Huang S Z, Hu M X, Wu X Q, Gao J M
Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, PRC.
Sci China B. 1994 Feb;37(2):170-6.
This study analyses the bovine SRY DNA sequence by direct sequencing procedure, followed by the designation of the PCR primers specific for bovine SRY. Using PCR amplification of bovine SRY gene, the embryo sex was determined. The results of the embryo sex identification were confirmed after the embryo transfer and pregnancies.
本研究通过直接测序程序分析牛SRY DNA序列,随后设计牛SRY特异性PCR引物。利用牛SRY基因的PCR扩增来确定胚胎性别。在胚胎移植和妊娠后对胚胎性别鉴定结果进行了确认。