Wang Feng, Yang Jinghe, Wu Xia, Wang Fei, Ding Honghong
Key Lab of Colloid and Interface Chemistry of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2007 Jun;67(2):385-90. doi: 10.1016/j.saa.2006.07.027. Epub 2006 Jul 28.
It is found that nucleic acid can enhance the resonance light scattering (RLS) enhancement effect of curcumin (CU) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB). The investigation indicates that in BR (pH 4.3) buffer, both the positive CTAB and negative yeast RNA (yRNA) combine and form a positive large association, then which is bound on the two carbon atoms of the carbonyls of CU through hydrogen bond and hydrophobic force and form CU-CTAB-yRNA ternary complex, resulting in the RLS enhancement of this system. Based on it, a sensitive method for determination of nucleic acids at ngml(-1) is established.
研究发现，在阳离子表面活性剂十六烷基三甲基溴化铵（CTAB）存在下，核酸可增强姜黄素（CU）的共振光散射（RLS）增强效应。研究表明，在BR（pH 4.3）缓冲液中，带正电的CTAB与带负电的酵母RNA（yRNA）结合形成带正电的大缔合物，然后通过氢键和疏水作用力与CU羰基的两个碳原子结合，形成CU-CTAB-yRNA三元复合物，导致该体系的RLS增强。基于此，建立了一种测定纳克每毫升（ng ml⁻¹）级核酸的灵敏方法。
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