银纳米粒子-姜黄素-十六烷基三甲基溴化铵-核酸体系的荧光增强及其分析应用。
Fluorescence enhancement of the silver nanoparticales--curcumin-cetyltrimethylammonium bromide-nucleic acids system and its analytical application.
机构信息
Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, China.
出版信息
J Fluoresc. 2010 Jul;20(4):843-50. doi: 10.1007/s10895-010-0627-z. Epub 2010 Mar 4.
It is found that silver nanoparticles (AgNPs) can further enhance the fluorescence intensity of curcumin (CU)-cetyltrimethylammonium bromide (CTAB)-nucleic acids and improve its anti-photobleaching activity. Under optimum conditions, the enhanced fluorescence intensity is proportion to the concentration of nucleic acids in the range of 2.0 x 10(-8)-1.0 x 10(-6) g mL(-1) for fish sperm DNA (fsDNA), 2.0 x 10(-8)-1.0 x 10(-6) g mL(-1) for calf thymus DNA (ctDNA), 1.0 x 10(-8)-1.0 x 10(-6) g mL(-1) for yeast RNA (yRNA), and their detection limits (S/N = 3) are 8.0 ng mL(-1), 10.5 ng mL(-1) and 5.8 ng mL(-1), respectively. This method is used for determining the concentration of DNA in actual sample with satisfactory results. The interaction mechanism is also studied.
研究发现，银纳米粒子（AgNPs）可以进一步增强姜黄素（CU）-十六烷基三甲基溴化铵（CTAB）-核酸的荧光强度，并提高其抗光漂白活性。在最佳条件下，增强的荧光强度与核酸的浓度成正比，在鱼精 DNA（fsDNA）的 2.0×10^(-8)-1.0×10^(-6)g/mL 范围内，小牛胸腺 DNA（ctDNA）的 2.0×10^(-8)-1.0×10^(-6)g/mL 范围内，酵母 RNA（yRNA）的 1.0×10^(-8)-1.0×10^(-6)g/mL 范围内，其检测限（S/N=3）分别为 8.0ng/mL、10.5ng/mL 和 5.8ng/mL。该方法用于测定实际样品中 DNA 的浓度，结果令人满意。还研究了相互作用机制。
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