Nedialkov Iu A, Drobyshevskaia E I, Klimchuk N D, Nesterenko V G, Tarasevich I V
Zh Mikrobiol Epidemiol Immunobiol. 1990 Aug(8):71-4.
The results of this investigation indicate that preparations obtained on the basis of monoclonal antibodies have proved to be suitable for the detection of R. prowazekii antigens in the natural carrier of typhus when used in all types of the enzyme immunoassay; of these, the assay made by the capture method has been found to possess the highest sensitivity. The testing of the sensitivity limits has shown that this method known as ELISA-mu-capture, i.e. ELISA carried out with the use of antibodies to the mu chain of human immunoglobulin, is capable of detecting the antigen in a dose of 0.5-1 ng. Preparations based on monoclonal antibodies to species-specific R. prowazekii antigen permit the identification of the causative agent of typhus in its natural carrier within 24 hours.
本次调查结果表明，基于单克隆抗体获得的制剂已被证明适用于在所有类型的酶免疫测定中检测斑疹伤寒自然携带者中的普氏立克次体抗原；其中，捕获法测定的灵敏度最高。灵敏度极限测试表明，这种被称为ELISA-mu-捕获的方法，即使用针对人免疫球蛋白μ链的抗体进行的ELISA，能够检测到剂量为0.5-1 ng的抗原。基于针对普氏立克次体种特异性抗原的单克隆抗体的制剂能够在24小时内鉴定出斑疹伤寒自然携带者中的病原体。
