非中和性人鼻病毒2型特异性单克隆抗体2G2附着于病毒RNA释放时发生最显著变化的区域。
Nonneutralizing human rhinovirus serotype 2-specific monoclonal antibody 2G2 attaches to the region that undergoes the most dramatic changes upon release of the viral RNA.
作者信息
Hewat Elizabeth A, Blaas Dieter
机构信息
Institut de Biologie Structurale Jean-Pierre Ebel, UMR 5057, CEA-CNRS-UJF, 41 rue Jules Horowitz, 38027 Grenoble, France.
出版信息
J Virol. 2006 Dec;80(24):12398-401. doi: 10.1128/JVI.01399-06. Epub 2006 Sep 27.
Abstract
The monoclonal antibody 2G2 has been used extensively for detection and quantification of structural changes of human rhinovirus serotype 2 during infection. It recognizes exclusively A and B subviral particles, not native virus. We have elucidated the basis of this selectivity by determining the footprint of 2G2. Since viral escape mutants obviously cannot be obtained, the structures of complexes between Fab fragments of 2G2 and 80S subviral B particles were determined by cryoelectron microscopy. The footprint of the antibody corresponds to the capsid region that we predicted would undergo the most dramatic changes upon RNA release.
摘要
单克隆抗体2G2已被广泛用于检测和定量人类鼻病毒2型在感染过程中的结构变化。它只识别A和B亚病毒颗粒，而不识别天然病毒。我们通过确定2G2的结合位点来阐明这种选择性的基础。由于显然无法获得病毒逃逸突变体，因此通过冷冻电子显微镜确定了2G2的Fab片段与80S亚病毒B颗粒之间复合物的结构。抗体的结合位点对应于衣壳区域，我们预测该区域在RNA释放时会发生最显著的变化。
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