从慢性感染泰勒氏鼠脑脊髓炎病毒的小鼠脊髓中分离出的传染性核糖核酸。
Infectious RNA isolated from the spinal cords of mice chronically infected with Theiler's murine encephalomyelitis virus.
作者信息
Libbey Jane E, Tsunoda Ikuo, Whitton J Lindsay, Fujinami Robert S
机构信息
Department of Neurology, University of Utah School of Medicine, 30 North 1900 East, 3R330 SOM, Salt Lake City, UT 84132-2305, USA.
出版信息
J Virol. 2007 Mar;81(6):3009-11. doi: 10.1128/JVI.01663-06. Epub 2006 Sep 27.
Abstract
The DA strain of Theiler's murine encephalomyelitis virus (TMEV) persistently infects cells of the spinal cord during the chronic phase of infection. Although in situ hybridization and reverse transcription-PCR have demonstrated the presence of viral RNA in the spinal cord, it has not been determined whether this RNA is infectious and, if so, how many PFU equivalents of virus the RNA can yield. In this study, we demonstrated that TMEV RNA isolated from the spinal cords of chronically infected mice is infectious and that there is at least 30-fold more infectious RNA than infectious virus in the spinal cords of these mice.
摘要
泰勒氏小鼠脑脊髓炎病毒（TMEV）的DA株在感染的慢性期持续感染脊髓细胞。尽管原位杂交和逆转录PCR已证明脊髓中存在病毒RNA，但尚未确定该RNA是否具有传染性，以及如果具有传染性，该RNA能产生多少个病毒蚀斑形成单位（PFU）当量的病毒。在本研究中，我们证明从慢性感染小鼠脊髓中分离的TMEV RNA具有传染性，并且在这些小鼠的脊髓中，感染性RNA比感染性病毒至少多30倍。
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