Sainz Bruno, Chisari Francis V
Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, SBR-10, La Jolla, CA 92037, USA.
J Virol. 2006 Oct;80(20):10253-7. doi: 10.1128/JVI.01059-06.
Dimethyl sulfoxide (DMSO) has been shown to induce the differentiation of primary hepatocytes in vitro. When actively dividing poorly differentiated human hepatoma-derived (Huh7) cells were cultured in the presence of 1% DMSO, cells became cytologically differentiated and transitioned into a nondividing state, characterized by the induction of hepatocyte-specific genes. Moreover, these cells were highly permissive for acute hepatitis C virus (HCV) infection, and persistent long term infection of these cultures could also be achieved. As HCV naturally replicates in highly differentiated nondividing human hepatocytes, this system may more accurately mimic the conditions under which HCV replicates in vivo than previous models using poorly differentiated rapidly dividing hepatoma cells.
二甲基亚砜（DMSO）已被证明可在体外诱导原代肝细胞分化。当在1% DMSO存在的情况下培养活跃分裂的低分化人肝癌来源（Huh7）细胞时，细胞在细胞学上发生分化并转变为非分裂状态，其特征是肝细胞特异性基因的诱导。此外，这些细胞对丙型肝炎病毒（HCV）急性感染高度敏感，并且这些培养物也可实现持续的长期感染。由于HCV自然地在高度分化的非分裂人肝细胞中复制，该系统可能比以前使用低分化快速分裂肝癌细胞的模型更准确地模拟HCV在体内复制的条件。
