Tauchi K, Tsutsumi Y, Yoshimura S, Watanabe K
Department of Pathology, Tokai University School of Medicine, Kanagawa, Japan.
Acta Pathol Jpn. 1990 Aug;40(8):574-80. doi: 10.1111/j.1440-1827.1990.tb01602.x.
Forty-six smooth muscle tumors, including 35 of gastrointestinal origin, were studied immunohistochemically for the localization of cytokeratin using a variety of monoclonal antibodies. In formalin-fixed, paraffin-embedded material, six of 40 leiomyomas, two of five leiomyosarcomas and one leiomyoblastoma were immunoreactive for cytokeratin in a few tumor cells. A proportion of non-neoplastic smooth muscle cells also exhibited a positive reaction. In fresh frozen sections of one gastric leiomyosarcoma, a high percentage of tumor cells were reactive with nine of the eleven anti-cytokeratin monoclonal antibodies examined. Cytokeratin-positive cytoplasmic filaments were further demonstrated by immunoelectron microscopy. By immunoblot analysis, an extract of this immunohistochemically cytokeratin-positive leiomyosarcoma showed a distinct band at the same position as an extract of pancreas, whereas no bands were seen in an extract of a cytokeratin-negative esophageal leiomyosarcoma. These immunohistochemical and immunoblotting findings indicate that a certain subset of smooth muscle neoplasms express genuine cytokeratin filaments.
对46例平滑肌肿瘤进行了免疫组织化学研究，其中包括35例胃肠道起源的肿瘤，使用多种单克隆抗体检测细胞角蛋白的定位。在福尔马林固定、石蜡包埋的材料中，40例平滑肌瘤中的6例、5例平滑肌肉瘤中的2例以及1例平滑肌瘤样细胞瘤的少数肿瘤细胞对细胞角蛋白呈免疫反应阳性。一部分非肿瘤性平滑肌细胞也呈阳性反应。在1例胃平滑肌肉瘤的新鲜冰冻切片中，在所检测的11种抗细胞角蛋白单克隆抗体中的9种抗体作用下，高比例的肿瘤细胞呈反应阳性。免疫电子显微镜进一步证实了细胞角蛋白阳性的细胞质细丝。通过免疫印迹分析，该免疫组织化学检测呈细胞角蛋白阳性的平滑肌肉瘤提取物在与胰腺提取物相同的位置显示出一条明显的条带，而细胞角蛋白阴性的食管平滑肌肉瘤提取物中未观察到条带。这些免疫组织化学和免疫印迹结果表明，平滑肌肿瘤的某一亚群表达真正的细胞角蛋白细丝。
