Mølbak Lars, Klitgaard Kirstine, Jensen Tim K, Fossi Marja, Boye Mette
Danish Institute for Food and Veterinary Research, Bülowsvej 27, DK-1790 Copenhagen V, Denmark.
J Clin Microbiol. 2006 Dec;44(12):4537-40. doi: 10.1128/JCM.01537-06. Epub 2006 Sep 27.
Laser capture microdissection in combination with fluorescent in situ hybridization was used to identify an unknown species of spirochetes from the pig colonic mucosa. The 16S rRNA gene was PCR amplified, and the closest related type strain was Treponema bryantiiT (90.1%). The spirochete, here named "Candidatus Treponema suis," was associated with colitis, including invasion of the surface epithelium as well as superficial parts of the mucosa.
结合荧光原位杂交的激光捕获显微切割技术被用于从猪结肠黏膜中鉴定一种未知的螺旋体物种。对16S rRNA基因进行了PCR扩增,与之亲缘关系最近的模式菌株是布氏密螺旋体T(90.1%)。这种螺旋体,此处命名为“猪密螺旋体暂定种”,与结肠炎相关,包括对表面上皮以及黏膜浅层的侵袭。