用于检测临床样本中K65R型人类免疫缺陷病毒1型逆转录酶突变病毒亚群的MultiCode-RTx实时荧光定量PCR系统
MultiCode-RTx real-time PCR system for detection of subpopulations of K65R human immunodeficiency virus type 1 reverse transcriptase mutant viruses in clinical samples.
作者信息
Svarovskaia Evguenia S, Moser Michael J, Bae Andrew S, Prudent James R, Miller Michael D, Borroto-Esoda Katyna
机构信息
Gilead Sciences, Inc., 4 University Place, 4611 University Drive, Durham, NC 27707, USA.
出版信息
J Clin Microbiol. 2006 Nov;44(11):4237-41. doi: 10.1128/JCM.01512-06. Epub 2006 Sep 27.
Abstract
We report a real-time PCR assay capable of detecting drug-resistant human immunodeficiency virus type 1 reverse transcriptase K65R mutant virus at a level of 0.5% in polymorphic patient plasma specimens. Fifty-three treatment-naïve and 20 treatment-experienced specimens were successfully genotyped with the new method. Results were in agreement with population sequencing and the labor-intensive single-genome sequencing method.
摘要
我们报告了一种实时聚合酶链反应（PCR）检测方法，该方法能够在多态性患者血浆样本中检测出耐药的1型人类免疫缺陷病毒逆转录酶K65R突变病毒，检测水平为0.5%。采用这种新方法对53例未经治疗和20例经治样本成功进行了基因分型。结果与群体测序以及耗时费力的单基因组测序方法一致。
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