Halvas Elias K, Aldrovandi Grace M, Balfe Peter, Beck Ingrid A, Boltz Valerie F, Coffin John M, Frenkel Lisa M, Hazelwood J Darren, Johnson Victoria A, Kearney Mary, Kovacs Andrea, Kuritzkes Daniel R, Metzner Karin J, Nissley Dwight V, Nowicki Marek, Palmer Sarah, Ziermann Rainer, Zhao Richard Y, Jennings Cheryl L, Bremer James, Brambilla Don, Mellors John W
Division of Infectious Diseases, Department of Medicine, University of Pittsburgh, S818 Scaife Hall, 3550 Terrace St., Pittsburgh, PA 15261, and Children's Hospital of Los Angeles, CA, USA.
J Clin Microbiol. 2006 Jul;44(7):2612-4. doi: 10.1128/JCM.00449-06.
We determined the abilities of 10 technologies to detect and quantify a common drug-resistant mutant of human immunodeficiency virus type 1 (lysine to asparagine at codon 103 of the reverse transcriptase) using a blinded test panel containing mutant-wild-type mixtures ranging from 0.01% to 100% mutant. Two technologies, allele-specific reverse transcriptase PCR and a Ty1HRT yeast system, could quantify the mutant down to 0.1 to 0.4%. These technologies should help define the impact of low-frequency drug-resistant mutants on response to antiretroviral therapy.
我们使用了一个包含突变体 - 野生型混合物(突变体比例从0.01%到100%)的盲测样本组,测定了10种技术检测和定量人类免疫缺陷病毒1型常见耐药突变体(逆转录酶第103位密码子赖氨酸突变为天冬酰胺)的能力。两种技术,即等位基因特异性逆转录酶PCR和Ty1HRT酵母系统,能够将突变体定量至0.1%至0.4%。这些技术应有助于明确低频耐药突变体对抗逆转录病毒治疗反应的影响。