Steinberger A, Heindel J J, Lindsey J N, Elkington J S, Sanborn B M, Steinberger E
Endocr Res Commun. 1975;2(3):261-72. doi: 10.3109/07435807509053853.
Methods for the isolation and culture of enriched populations of Sertoli cells from 20-60 day old rats are described. The identity of the Sertoli cells was verified by bright light and electron microscopy. Freshly isolated Sertoli cells specifically bound follicle stimulating hormone (FSH) but not luteinizing hormone (LH) and responded to FSH stimulation with dramatic increase in cyclic AMP level. Isolated Sertoli cells, maintained in culture for 11 days, showed no evidence of proliferation but retained their characteristic ultrastructural features and FSH binding ability. Incubation of cultured cells with FSH resulted in a significant stimulation of cyclic AMP and androgen binding protein (ABP). Since the freshly isolated or cultured cells were predominantly (greater than 80%) Sertoli cells, these results provide direct evidence that the Sertoli cells represent a primary target site for FSH activity in the testes. The culture method also provides a valuable in vitro model for the study of chronic effects of various agents on the Sertoli cell.
本文描述了从20至60日龄大鼠中分离和培养富集支持细胞群体的方法。通过明视野显微镜和电子显微镜对支持细胞的身份进行了验证。新鲜分离的支持细胞特异性结合促卵泡激素（FSH），但不结合促黄体生成素（LH），并且对FSH刺激有反应，细胞内环磷酸腺苷（cAMP）水平显著升高。分离的支持细胞在培养11天中未显示增殖迹象，但保留了其特征性超微结构特征和FSH结合能力。用FSH孵育培养细胞导致cAMP和雄激素结合蛋白（ABP）受到显著刺激。由于新鲜分离或培养的细胞主要（超过80%）是支持细胞，这些结果提供了直接证据，表明支持细胞是睾丸中FSH活性的主要靶位点。该培养方法还为研究各种药物对支持细胞的慢性影响提供了有价值的体外模型。
