Fritz I B, Griswold M D, Louis B G, Dorrington J H
Mol Cell Endocrinol. 1976 Aug-Sep;5(3-4):289-94. doi: 10.1016/0303-7207(76)90090-3.
The effects of cholera toxin on the responses of cultured Sertoli cells were compared with those elicited by follicle-stimulating hormone (FSH), and N6O2'-dibutyryl-3',5'-cyclic AMP (bu2cAMP). Addition of FSH or cholera toxin increased cAMP levels. Subsequently there was greater rates of conversion of testosterone to 17beta-estradiol, formation of androgen-binding protein (ABP), and incorporation of [3H]thymidine into DNA by Sertoli cells prepared from testes of immature rats and cultured in the presence of either FSH or cholera toxin. Addition of bu2-cAMP also resulted in enhanced rates of formation of ABP, synthesis of 17beta-estradiol and synthesis of DNA. Cholera toxin and bu2-cAMP elicited changes in morphology of cultured Sertoli cells indistinguishable from those following FSH addition. It is concluded that elevated intracellular cAMP levels can duplicate known actions of FSH on cultured Sertoli cells, but the possible obligatory role of cAMP in mediating FSH actions remains to be evaluated.
将霍乱毒素对培养的支持细胞反应的影响与促卵泡激素（FSH）和N6O2'-二丁酰-3',5'-环磷酸腺苷（bu2cAMP）所引发的影响进行了比较。添加FSH或霍乱毒素会增加环磷酸腺苷（cAMP）水平。随后，在由未成熟大鼠睾丸制备并在FSH或霍乱毒素存在下培养的支持细胞中，睾酮向17β-雌二醇的转化速率、雄激素结合蛋白（ABP）的形成以及[3H]胸腺嘧啶核苷掺入DNA的速率均更高。添加bu2-cAMP也导致ABP形成速率、17β-雌二醇合成以及DNA合成增加。霍乱毒素和bu2-cAMP引起的培养支持细胞形态变化与添加FSH后的变化无法区分。结论是，细胞内cAMP水平升高可复制FSH对培养支持细胞的已知作用，但cAMP在介导FSH作用中可能的必需作用仍有待评估。
