Duk Maria, Lisowska Elwira
Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolf Weigl St. 12, 53-114, Wroclaw, Poland.
Glycoconj J. 2006 Nov;23(7-8):585-90. doi: 10.1007/s10719-006-8188-8.
Rare polyagglutinable NOR erythrocytes contain unusual globoside extention products terminating with a Galalpha1-4GalNAcbeta1-3Gal- unit. This trisaccharide epitope is recognized by recently characterized antibodies naturally occurring in most human sera (Duk et al., Glycobiology, 15, 109, 2005). These antibodies represent two major types of fine specificity. All these antibodies are most strongly inhibited by Galalpha1-4GalNAcbeta1-3Gal (NOR-tri), and weakly by Galalpha1-4Gal. However, the type 1 antibodies are strongly inhibited by Galalpha1-4Galbeta1-3Gal-R and weakly by Galalpha1-4GalNAc, while the type 2 antibodies show the opposite reactivities with these two oligosaccharides. Similar antibodies have now been found in horse, rabbit and pig sera. The antibodies were purified from animal sera by affinity chromatography on Galalpha1-4GalNAcbeta1-3Gal-human serum albumin(HSA)-Sepharose 4B conjugate. The specificity of the antibodies was determined by binding to ELISA plates coated with several alpha-galactosylated oligosaccharide-polyacrylamide (PAA) or -HSA conjugates and by inhibition with synthetic oligosaccharides. The purified antibodies bound specifically to conjugates containing NOR-tri. The inhibition of binding showed that the animal sera also contain two types of anti-NOR antibodies: type 2 was found in the horse serum, and a mixture of both types was present in rabbit and pig serum. These results indicate that anti-NOR, a new and distinct kind of anti-alphaGal antibody, are present in animal sera and show similar specificties and diversity as their counterparts found in human sera.
罕见的多凝集性NOR红细胞含有以Galα1-4GalNAcβ1-3Gal-单元结尾的异常糖苷延伸产物。这种三糖表位可被最近鉴定出的、天然存在于大多数人血清中的抗体识别（Duk等人，《糖生物学》，15卷，109页，2005年）。这些抗体代表两种主要的精细特异性类型。所有这些抗体都被Galα1-4GalNAcβ1-3Gal（NOR-三糖）强烈抑制，被Galα1-4Gal微弱抑制。然而，1型抗体被Galα1-4Galβ1-3Gal-R强烈抑制，被Galα1-4GalNAc微弱抑制，而2型抗体与这两种寡糖的反应性则相反。现已在马、兔和猪的血清中发现了类似的抗体。通过在Galα1-4GalNAcβ1-3Gal-人血清白蛋白（HSA）-琼脂糖4B偶联物上进行亲和层析，从动物血清中纯化出这些抗体。通过与包被有几种α-半乳糖基化寡糖-聚丙烯酰胺（PAA）或-HSA偶联物的ELISA板结合以及用合成寡糖进行抑制，来确定抗体的特异性。纯化后的抗体与含有NOR-三糖的偶联物特异性结合。结合抑制表明动物血清中也含有两种抗NOR抗体：2型存在于马血清中，兔和猪血清中则同时存在两种类型的混合物。这些结果表明，抗NOR是一种新的、独特的抗αGal抗体，存在于动物血清中，并且与在人血清中发现的对应抗体具有相似的特异性和多样性。
