Terrier Bénédicte, Courtois Didier, Hénault Nicolas, Cuvier Arnaud, Bastin Maryse, Aknin Aziz, Dubreuil Julien, Pétiard Vincent
Centre de Recherche & Développement Nestlé-Tours, 101, Avenue Gustave Eiffel, BP 49716, 37097 Tours Cedex 2, France.
Biotechnol Bioeng. 2007 Apr 1;96(5):914-23. doi: 10.1002/bit.21187.
The present article describes two novel flexible plastic-based disposable bioreactors. The first one, the WU bioreactor, is based on the principle of a wave and undertow mechanism that provides agitation while offering convenient mixing and aeration to the plant cell culture contained within the bioreactor. The second one is a high aspect ratio bubble column bioreactor, where agitation and aeration are achieved through the intermittent generation of large diameter bubbles, "Taylor-like" or "slug bubbles" (SB bioreactor). It allows an easy volume increase from a few liters to larger volumes up to several hundred liters with the use of multiple units. The cultivation of tobacco and soya cells producing isoflavones is described up to 70 and 100 L working volume for the SB bioreactor and WU bioreactor, respectively. The bioreactors being disposable and pre-sterilized before use, cleaning, sterilization, and maintenance operations are strongly reduced or eliminated. Both bioreactors represent efficient and low cost cell culture systems, applicable to various cell cultures at small and medium scale, complementary to traditional stainless-steel bioreactors.
本文介绍了两种新型的基于柔性塑料的一次性生物反应器。第一种是WU生物反应器，它基于波浪和回流机制的原理，在为生物反应器内的植物细胞培养物提供方便的混合和通气的同时实现搅拌。第二种是高径比鼓泡塔生物反应器，其中通过间歇性产生大直径气泡（“类泰勒”或“弹状气泡”，即SB生物反应器）来实现搅拌和通气。使用多个单元时，它可以轻松地将体积从几升增加到几百升的更大体积。分别描述了在SB生物反应器和WU生物反应器中，工作体积达70升和100升时烟草和大豆细胞生产异黄酮的培养情况。这些生物反应器是一次性的，在使用前经过预灭菌，大大减少或消除了清洁、灭菌和维护操作。这两种生物反应器均代表高效且低成本的细胞培养系统，适用于中小规模的各种细胞培养，是传统不锈钢生物反应器的补充。
