Department of Molecular Biotechnology and Genetics, University of Lodz, 90-237 Lodz, Poland.
Department of Economic Informatics, University of Lodz, 90-214 Lodz, Poland.
Molecules. 2022 Jan 25;27(3):795. doi: 10.3390/molecules27030795.
In recent years, there has been a considerable increase in interest in the use of transgenic plants as sources of valuable secondary metabolites or recombinant proteins. This has been facilitated by the advent of genetic engineering technology with the possibility for direct modification of the expression of genes related to the biosynthesis of biologically active compounds. A wide range of research projects have yielded a number of efficient plant systems that produce specific secondary metabolites or recombinant proteins. Furthermore, the use of bioreactors allows production to be increased to industrial scales, which can quickly and cheaply deliver large amounts of material in a short time. The resulting plant production systems can function as small factories, and many of them that are targeted at a specific operation have been patented. This review paper summarizes the key research in the last ten years regarding the use of transgenic plants as small, green biofactories for the bioreactor-based production of secondary metabolites and recombinant proteins; it simultaneously examines the production of metabolites and recombinant proteins on an industrial scale and presents the current state of available patents in the field.
近年来，人们对利用转基因植物作为有价值的次生代谢物或重组蛋白的来源产生了浓厚的兴趣。这得益于基因工程技术的出现，使得与生物活性化合物生物合成相关的基因表达的直接修饰成为可能。许多广泛的研究项目已经产生了许多有效的植物系统，这些系统可以产生特定的次生代谢物或重组蛋白。此外，生物反应器的使用允许生产增加到工业规模，可以在短时间内快速廉价地提供大量材料。由此产生的植物生产系统可以作为小型工厂运作，其中许多针对特定操作的系统已经获得专利。本文综述了过去十年中关于利用转基因植物作为小型绿色生物工厂，通过生物反应器生产次生代谢物和重组蛋白的关键研究；同时考察了在工业规模上生产代谢物和重组蛋白的情况，并介绍了该领域现有专利的现状。
