Hong Joon Pio, Kim Yun Wha, Jung Heun Don, Jung Kwon Il
Department of Plastic and Reconstructive Surgery, University of Ulsan, College of Medicine, Asan Medical Center, Seoul, Korea.
Int Wound J. 2006 Jun;3(2):123-30. doi: 10.1111/j.1742-4801.2006.00187.x.
Epidermal growth factor (EGF) is a potent stimulant of epithelialisation. However, topical application of EGF to achieve facilitated re-epithelialisation in partial thickness wounds has been controversial. A total of 10 pigs, each with eight 4 x 4 cm partial thickness wounds, were treated twice a day for 10 days to observe the effect of human recombinant EGF in concentrations of 0.1, 1, 5, 10, 25 ug/g, vehicle only and two controls. The control and the vehicle-only wounds each demonstrated 100% healing time (HT100) of 9.31 +/- 1.34 and 8.5 +/- 1.12 while the wounds treated with EGF ointment with concentrations of 0.1 (HT100 = 6.4 +/- 0.71), 1 (HT100 = 5.2 +/- 0.63), 5 (HT100 = 5.8 +/- 0.85), 10 (HT100 = 7.1 +/- 1.45) and 25 ug/g (HT100 = 7.4 + 0.57) demonstrated significant reduction in time to achieve re-epithelialisation. Among the EGF-treated wounds, the wounds treated with EGF concentrations of 1 and 5 ug/g achieved the fastest re-epithelialisation with evidence of substantial increase in basal keratinocyte activity observed through Ki-67 activity. In conclusion, this article demonstrates the efficacy of human recombinant EGF in facilitating re-epithelialisation of partial thickness wounds with the most efficient healing found in EGF concentrations of 1 and 5 ug/g.
表皮生长因子（EGF）是上皮形成的一种强效刺激物。然而，局部应用EGF以促进部分厚度伤口的再上皮形成一直存在争议。总共10头猪，每头猪有8个4×4厘米的部分厚度伤口，每天治疗两次，持续10天，以观察浓度为0.1、1、5、10、25微克/克的人重组EGF、仅用赋形剂以及两个对照的效果。对照伤口和仅用赋形剂的伤口的100%愈合时间（HT100）分别为9.31±1.34和8.5±1.12，而用浓度为0.1（HT100 = 6.4±0.71）、1（HT100 = 5.2±0.63）、5（HT100 = 5.8±0.85）、10（HT100 = 7.1±1.45）和25微克/克（HT100 = 7.4 + 0.57）的EGF软膏治疗的伤口，实现再上皮形成的时间显著缩短。在接受EGF治疗的伤口中，用浓度为1和5微克/克的EGF治疗的伤口实现再上皮形成的速度最快，通过Ki-67活性观察到基底角质形成细胞活性大幅增加。总之，本文证明了人重组EGF在促进部分厚度伤口再上皮形成方面的有效性，其中浓度为1和5微克/克的EGF愈合效率最高。
