Wahl R, Lau S, Maasch H J, Wahn U
Allergopharma Joachim Ganzer KG, Research and Development of Allergen Extract Preparations, Reinbek bei Hamburg, FRG.
Int Arch Allergy Appl Immunol. 1990;92(2):168-74. doi: 10.1159/000235209.
We investigated sera of 12 patients with IgE-mediated hypersensitivity reactions to potato, using different in vitro methods. Radioallergosorbent test classes 2-4 (7.6-46.5% binding) were measured with potato allergen disks. Immunoblot detected allergen bands with an isoelectric point range of approximately 4.5-5.2 and mainly in a molecular weight range between 16.00 and 30.00 kD. But also at molecular weights of 43.00 and 65.00 kD allergen bands (IgE specificities) could be detected. Histamine release assay performed with isolated fractions of the potato extract showed a great individual variation and positive results of fractions of molecular weights between 10.00 and 80.00 kD. From our data we conclude that raw potato is a 'multiallergenic' vegetable.
我们使用不同的体外方法，对12例对马铃薯产生IgE介导的超敏反应患者的血清进行了研究。使用马铃薯过敏原盘测定放射性变应原吸附试验2-4级（结合率7.6-46.5%）。免疫印迹检测到等电点范围约为4.5-5.2且主要分子量范围在16.00至30.00kD之间的过敏原条带。但在分子量为43.00和65.00kD时也可检测到过敏原条带（IgE特异性）。用马铃薯提取物的分离组分进行的组胺释放试验显示个体差异很大，分子量在10.00至80.00kD之间的组分呈阳性结果。根据我们的数据，我们得出结论，生马铃薯是一种“多过敏原性”蔬菜。
