Haarasilta S, Oura E
Eur J Biochem. 1975 Mar 3;52(1):1-7. doi: 10.1111/j.1432-1033.1975.tb03966.x.
The anaplerotic and gluconeogenetic metabolism of baker's yeast was studied at the enzymatic level during glucose-ethanol diauxic growth in the presence and absence of aspartate. Of the two possible anaplerotic systems, only the pyruvate carboxylase by-pass was present during the whole growth process. The second system, the glyoxylate by-pass (isocitrate lyase as the indicator), like the specific enzymes of the gluconeogenetic metabolism, phosphoenolpyruvate carboxykinase and hexosediphosphatase began to appear only after the glucose had been consumed. The addition of glucose during the growth phase based on ethanol effected a rapid disappearance of phosphoenolpyruvate carboxykinase and hexosediphosphatase activities. The activity of pyruvate carboxylase decreased when the growth medium was supplied with asparate. The presence of aspartate had no effect on the activities of the other enzymes studied.
在有天冬氨酸和无天冬氨酸存在的情况下，对面包酵母在葡萄糖 - 乙醇双相生长过程中的回补和糖异生代谢进行了酶水平的研究。在两种可能的回补系统中，只有丙酮酸羧化酶支路在整个生长过程中存在。第二个系统，乙醛酸支路（以异柠檬酸裂解酶为指标），与糖异生代谢的特定酶磷酸烯醇丙酮酸羧激酶和己糖二磷酸酶一样，只有在葡萄糖消耗完后才开始出现。在以乙醇为基础的生长阶段添加葡萄糖会使磷酸烯醇丙酮酸羧激酶和己糖二磷酸酶的活性迅速消失。当生长培养基中添加天冬氨酸时，丙酮酸羧化酶的活性降低。天冬氨酸的存在对所研究的其他酶的活性没有影响。
