Haarasilta S, Taskinen L
Arch Microbiol. 1977 May 13;113(1-2):159-61. doi: 10.1007/BF00428597.
The subcellular location of hexose diphosphatase, phosphoenolpyruvate carboxykinase and pyruvate carboxylase in baker's yeast (Saccharomyces cerevisiae) was investigated by density gradient centrifugation of spheroplast lysates obtained by osmotic shock treatment of spheroplasts and centrifugation for 10000 g x min. On the evidence obtained from zonal separations these three enzymes of gluconeogenesis are most probably located in the soluble cytosol.
通过对经渗透休克处理的球状体进行离心（10000g×分钟）后获得的球状体裂解物进行密度梯度离心，研究了面包酵母（酿酒酵母）中己糖二磷酸酶、磷酸烯醇式丙酮酸羧激酶和丙酮酸羧化酶的亚细胞定位。根据区域分离获得的证据，这三种糖异生酶很可能位于可溶性胞质溶胶中。
