Schumacher Karin
ZMBP-Plant Physiology, Universität Tübingen, Auf der Morgenstelle 1, 72076 Tübingen, Germany.
Curr Opin Plant Biol. 2006 Dec;9(6):595-600. doi: 10.1016/j.pbi.2006.09.001. Epub 2006 Sep 27.
pH-homeostasis in the endomembrane system requires the activity of proton-pumps. In animals, the progressive acidification of compartments along the endocytic and secretory pathways is critical for protein sorting and vesicle trafficking, and is achieved by the activity of the vacuolar H(+)-ATPase (V-ATPase). Plants have an additional endomembrane pump, the vacuolar H(+)-pyrophosphatase (V-PPase), and previous research was largely focused on the respective functions of the two pumps in secondary active transport across the tonoplast. Recent approaches, including reverse genetics, have not only provided evidence that both enzymes play unique and essential roles but have also highlighted the important functions of the two proton pumps in endocytic and secretory trafficking.
内膜系统中的pH稳态需要质子泵的活性。在动物中，沿着内吞和分泌途径的区室逐渐酸化对于蛋白质分选和囊泡运输至关重要，这是通过液泡H(+)-ATP酶（V-ATP酶）的活性实现的。植物还有另一种内膜泵，即液泡H(+)-焦磷酸酶（V-PP酶），先前的研究主要集中在这两种泵在跨液泡膜的次级主动运输中的各自功能。包括反向遗传学在内的最新研究方法不仅提供了证据表明这两种酶都发挥着独特且必不可少的作用，还突出了这两种质子泵在内吞和分泌运输中的重要功能。
