Heidelberg Institute for Plant Sciences (HIP), Universität Heidelberg, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany.
Curr Opin Plant Biol. 2010 Dec;13(6):724-30. doi: 10.1016/j.pbi.2010.07.003. Epub 2010 Aug 26.
About 30 years ago seminal reports of anion-sensitive proton-pumping activity associated with microsomal membranes initiated research on the plant vacuolar-type H(+)-ATPase (V-ATPase, VHA). Since, it has been firmly established that these complex molecular machines are essential for what can be defined as cellular logistics. In a eukaryotic cell, the flow of goods between compartments is achieved either by protein-mediated membrane transport or via vesicular trafficking. Over the past years, it has become increasingly clear that V-ATPases do not only energize secondary active transport but are also important regulators of membrane trafficking.
大约 30 年前，与微粒体膜相关的阴离子敏感质子泵活性的开创性报告启动了对植物液泡型 H(+) -ATP 酶（V-ATPase，VHA）的研究。从那时起，已经明确这些复杂的分子机器对于可以定义为细胞物流的过程是必不可少的。在真核细胞中，隔室之间的物质流通过蛋白介导的膜运输或通过小泡运输来实现。在过去的几年中，越来越明显的是，V-ATPases 不仅为次级主动运输提供能量，而且还是膜运输的重要调节剂。
