Rinaudo Paolo F, Giritharan Gnanaratnam, Talbi Said, Dobson Anthony T, Schultz Richard M
Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Fertil Steril. 2006 Oct;86(4 Suppl):1252-65, 1265.e1-36. doi: 10.1016/j.fertnstert.2006.05.017.
To compare the effects of atmospheric and physiologic oxygen concentrations on the global patterns of gene expression during mouse preimplantation development.
Comparative analysis of in vivo-produced and in vitro-produced embryos.
Research laboratory.
PATIENT(S): None.
INTERVENTION(S): Control embryos at the blastocyst stage that developed in vivo were collected from uteri. Experimental embryos were obtained at the zygote stage and cultured to the blastocyst stage in Whitten's medium or KSOM medium with amino acids under 20% oxygen (atmospheric) or 5% oxygen (physiologic).
MAIN OUTCOME MEASURE(S): Embryo development, cell number, and gene expression assayed by microarray technology.
RESULT(S): Low (physiologic) oxygen concentration is associated with faster embryo development and increased cell number. In addition, there are marked perturbations in the global pattern of gene expression, as assessed by oligonucleotide microarray, after culture in 20% oxygen as compared with 5% oxygen.
CONCLUSION(S): Culture in low oxygen is associated with fewer perturbations in the global pattern of gene expression and more closely resembles that of the in vivo control embryos. These findings provide rationale for culturing human embryos in the presence of 5%, rather than 20%, oxygen.
比较大气氧浓度和生理氧浓度对小鼠植入前发育过程中基因表达全局模式的影响。
体内产生和体外产生胚胎的比较分析。
研究实验室。
无。
从子宫中收集体内发育至囊胚阶段的对照胚胎。实验胚胎在合子期获得，并在含氨基酸的惠顿培养基或KSOM培养基中于20%氧气（大气氧浓度）或5%氧气（生理氧浓度）条件下培养至囊胚阶段。
通过微阵列技术检测胚胎发育、细胞数量和基因表达。
低（生理）氧浓度与更快的胚胎发育和增加的细胞数量相关。此外，与5%氧气培养相比，在20%氧气培养后，通过寡核苷酸微阵列评估发现基因表达全局模式存在明显扰动。
低氧培养与基因表达全局模式的扰动较少相关，并且更类似于体内对照胚胎。这些发现为在5%而非20%氧气存在下培养人类胚胎提供了理论依据。
