Plachouras Diamantis, Routsi Christina, Giamarellos-Bourboulis Evangelos J, Spyridaki Ekaterini, Andrianakis Ilias, Metzelopoulos Spyridon, Tsaganos Thomas, Floros Ioannis, Douzinas Emmanuel E, Armaganidis Apostolos, Roussos Charis, Giamarellou Helen
Fourth Department of Internal Medicine, University of Athens Medical School, Greece.
Scand J Infect Dis. 2006;38(10):909-15. doi: 10.1080/00365540600786523.
The role of blood monocytes in the secretion of soluble triggering receptor expressed on myeloiod cells (sTREM-1) was studied in 90 patients with septic syndrome due to ventilator-associated pneumonia. Blood monocytes were isolated on 7 consecutive d after initiation of symptoms. Monocytes were incubated in the absence or presence of LPS and concentrations of sTREM-1 and TNFalpha in cell supernatants and serum were estimated by an enzyme-immunoassay. sTREM-1 and TNFalpha were consistently present at detectable levels in the cell supernatants. LPS induced increased levels of TNFalpha but not of sTREM-1. Supernatants recovered from monocytes on d 1 showed levels of sTREM-1 higher than those recovered on any of the following 6 d (p<0.05); these levels were higher in non-survivors than in survivors. Supernatants recovered from monocytes on d 1 of patients with severe sepsis had elevated concentrations of sTREM-1 compared to patients with septic shock and similar to patients with sepsis. A negative correlation was found between levels of sTREM-1 in the cell supernatants and the percentage of apoptotic monocytes. In essence, the above results suggest that monocytes contribute to the production of sTREM-1 in the event of septic syndrome.
在90例因呼吸机相关性肺炎导致脓毒症综合征的患者中，研究了血液单核细胞在髓系细胞表面表达的可溶性触发受体（sTREM-1）分泌中的作用。症状出现后连续7天分离血液单核细胞。将单核细胞在不存在或存在脂多糖（LPS）的情况下孵育，并用酶免疫测定法估计细胞上清液和血清中sTREM-1和肿瘤坏死因子α（TNFα）的浓度。sTREM-1和TNFα在细胞上清液中始终以可检测水平存在。LPS诱导TNFα水平升高，但不诱导sTREM-1水平升高。第1天从单核细胞中回收的上清液中sTREM-1水平高于随后6天中任何一天回收的上清液（p<0.05）；非幸存者中的这些水平高于幸存者。与脓毒性休克患者相比，严重脓毒症患者第1天从单核细胞中回收的上清液中sTREM-1浓度升高，与脓毒症患者相似。在细胞上清液中sTREM-1水平与凋亡单核细胞百分比之间发现负相关。实质上，上述结果表明，在脓毒症综合征发生时，单核细胞有助于sTREM-1的产生。
