Dimopoulou I, Orfanos S E, Pelekanou A, Kotanidou A, Livaditi O, Augustatou C, Zervou M, Douka E, Theodorakopoulou M, Karagianni V, Douzinas E, Armaganidis A, Giamarellos-Bourboulis E J
2nd Department of Critical Care Medicine, University of Athens, Greece.
Inflamm Res. 2009 Mar;58(3):127-32. doi: 10.1007/s00011-008-7039-4.
To describe the concentrations of sTREM-1 in patients with sepsis and to explore the effects of their serum on the expression of TREM-1 on U937 monocytes.
Blood was sampled at regular time intervals in 56 patients with sepsis. Concentrations of tumour necrosis factor-alpha (TNFalpha), interleukin-1beta (IL-1alpha), IL-6, IL-8, IL-10 and IL-12p70 and sTREM-1 were measured. U937 monocytes were incubated in the presence of serum at sepsis onset.
Median sTREM-1 concentration on day 1 for patients with septic shock was 915 pg/ml and 228.5 pg/ml for those without shock (p = 0.002). TNFalpha, IL-1alpha, IL-6, IL-8 and IL-10 did not differ between them. A positive correlation was found between changes of sTREM-1 and SOFA scores from day 1 to 7. Sera of patients with septic shock evoked a significant increase of the expression of TREM-1. The concentrations of TNFalpha and IL-8 in supernatants increased only after stimulating with sera of patients without shock, but not after stimulating with sera of patients with shock.
Levels of sTREM-1 correlated with sepsis severity. sTREM-1 is considerably higher in patients with shock compared to patients without shock. The serum of shocked patients could stimulate the expression of TREM-1 on U937 monocytes.
描述脓毒症患者可溶性髓系细胞触发受体-1（sTREM-1）的浓度，并探讨其血清对U937单核细胞上TREM-1表达的影响。
对56例脓毒症患者定期采血。检测肿瘤坏死因子-α（TNFα）、白细胞介素-1β（IL-1α）、IL-6、IL-8、IL-10、IL-12p70和sTREM-1的浓度。将U937单核细胞在脓毒症发作时的血清存在下进行孵育。
感染性休克患者第1天sTREM-1浓度中位数为915 pg/ml，无休克患者为228.5 pg/ml（p = 0.002）。TNFα、IL-1α、IL-6、IL-8和IL-10在两者之间无差异。发现第1天至第7天sTREM-1的变化与序贯器官衰竭评估（SOFA）评分呈正相关。感染性休克患者的血清可引起TREM-1表达显著增加。仅在用无休克患者的血清刺激后，上清液中TNFα和IL-8的浓度才升高，而用休克患者的血清刺激后则未升高。
sTREM-1水平与脓毒症严重程度相关。与无休克患者相比，休克患者的sTREM-1水平显著更高。休克患者的血清可刺激U937单核细胞上TREM-1的表达。
