Marra Roberta, Ambrosino Patrizia, Carbone Virginia, Vinale Francesco, Woo Sheridan L, Ruocco Michelina, Ciliento Rosalia, Lanzuise Stefania, Ferraioli Simona, Soriente Ida, Gigante Sarah, Turrà David, Fogliano Vincenzo, Scala Felice, Lorito Matteo
Dipartimento di Arboricoltura, Botanica e Patologia Vegetale, Università degli Studi di Napoli Federico II, Portici (NA), Italy.
Curr Genet. 2006 Nov;50(5):307-21. doi: 10.1007/s00294-006-0091-0. Epub 2006 Aug 29.
The main molecular factors involved in the complex interactions occurring between plants (bean), two different fungal pathogens (Botrytis cinerea, Rhizoctonia solani) and an antagonistic strain of the genus Trichoderma were investigated. Two-dimensional (2-D) electrophoresis was used to analyze separately collected proteomes from each single, two- or three-partner interaction (i.e., plant, pathogenic and antagonistic fungus alone and in all possible combinations). Differential proteins were subjected to mass spectrometry and in silico analysis to search for homologies with known proteins. In the plant proteome, specific pathogenesis-related proteins and other disease-related factors (i.e., potential resistance genes) seem to be associated with the interaction with either one of the two pathogens and/or T. atroviride. This finding is in agreement with the demonstrated ability of Trichoderma spp. to induce systemic resistance against various microbial pathogens. On the other side, many differential proteins obtained from the T. atroviride interaction proteome showed interesting homologies with a fungal hydrophobin, ABC transporters, etc. Virulence factors, like cyclophilins, were up-regulated in the pathogen proteome during the interaction with the plant alone or with the antagonist too. We isolated and confidently identified a large number of protein factors associated to the multi-player interactions examined.
研究了植物（豆类）、两种不同的真菌病原体（灰葡萄孢、立枯丝核菌）和木霉属的一种拮抗菌株之间复杂相互作用中涉及的主要分子因素。二维（2-D）电泳用于分析从每种单一、二元或三元相互作用（即植物、致病真菌和拮抗菌单独以及所有可能组合）中分别收集的蛋白质组。对差异蛋白进行质谱分析和计算机分析，以寻找与已知蛋白的同源性。在植物蛋白质组中，特定的病程相关蛋白和其他与疾病相关的因素（即潜在的抗性基因）似乎与与两种病原体之一和/或绿色木霉的相互作用有关。这一发现与木霉属诱导对各种微生物病原体的系统抗性的能力相一致。另一方面，从绿色木霉相互作用蛋白质组中获得的许多差异蛋白与真菌疏水蛋白、ABC转运蛋白等显示出有趣的同源性。在与植物单独或与拮抗剂相互作用期间，诸如亲环蛋白等毒力因子在病原体蛋白质组中上调。我们分离并可靠地鉴定了大量与所研究的多因素相互作用相关的蛋白质因子。
