短型粘蛋白6等位基因与幽门螺杆菌感染相关。
Short mucin 6 alleles are associated with H pylori infection.
作者信息
Nguyen Thai V, Janssen Marcel, Gritters Paulien, te Morsche René H M, Drenth Joost P H, van Asten Henri, Laheij Robert J F, Jansen Jan B M J
机构信息
Department of Gastroenterology, Can Tho University of Medicine and Pharmacy, Viet Nam.
出版信息
World J Gastroenterol. 2006 Oct 7;12(37):6021-5. doi: 10.3748/wjg.v12.i37.6021.
AIM
To investigate the relationship between mucin 6 (MUC6) VNTR length and H pylori infection.
METHODS
Blood samples were collected from patients visiting the Can Tho General Hospital for upper gastrointestinal endoscopy. DNA was isolated from whole blood, the repeated section was cut out using a restriction enzyme (Pvu II) and the length of the allele fragments was determined by Southern blotting. H pylori infection was diagnosed by (14)C urea breath test. For analysis, MUC6 allele fragment length was dichotomized as being either long (> 13.5 kbp) or short (< or = 13.5 kbp) and patients were classified according to genotype [long-long (LL), long-short (LS), short-short (SS)].
RESULTS
160 patients were studied (mean age 43 years, 36% were males, 58% H pylori positive). MUC6 Pvu II-restricted allele fragment lengths ranged from 7 to 19 kbp. Of the patients with the LL, LS, SS MUC6 genotype, 43% (24/56), 57% (25/58) and 76% (11/46) were infected with H pylori, respectively (P = 0.003).
CONCLUSION
Short MUC6 alleles are associated with H pylori infection.
目的
研究黏蛋白6（MUC6）可变数目串联重复序列（VNTR）长度与幽门螺杆菌感染之间的关系。
方法
收集到芹苴综合医院接受上消化道内镜检查患者的血样。从全血中提取DNA，使用限制性内切酶（Pvu II）切下重复片段，通过Southern印迹法测定等位基因片段的长度。采用碳-14尿素呼气试验诊断幽门螺杆菌感染。分析时，将MUC6等位基因片段长度分为长片段（> 13.5 kbp）或短片段（≤ 13.5 kbp），并根据基因型[长-长（LL）、长-短（LS）、短-短（SS）]对患者进行分类。
结果
对160例患者进行了研究（平均年龄43岁，36%为男性，58%幽门螺杆菌阳性）。MUC6经Pvu II酶切后的等位基因片段长度范围为7至19 kbp。LL、LS、SS MUC6基因型患者中，幽门螺杆菌感染率分别为43%（24/56）、57%（25/58）和76%（11/46）（P = 0.003）。
结论
MUC6短等位基因与幽门螺杆菌感染相关。
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