Williams N E, Walker S C, Reeves D E, Sherrer E, Galvin J M, Polejaeva I, Rampacek G, Benyshek L, Christenson R K, Graves W M, Pratt S L
ViaGen, Inc., Austin, Texas 78727, USA.
Cloning Stem Cells. 2006 Fall;8(3):130-9. doi: 10.1089/clo.2006.8.130.
This study compares the reproductive performance of boars produced by somatic cell nuclear transfer versus conventional breeding. Two different genotypes were selected for comparison: terminal cross line 1 (TX1) and terminal cross line 2 (TX2). The boars selected for comparison from TX1 were three cloned boars, produced by somatic cell nuclear transfer and the conventionally produced progenitor of the clones. The boars selected for comparison from TX2 were a cloned boar produced by somatic cell nuclear transfer and two conventionally produced half sibling boars that were offspring of the progenitor of the clone. Semen from each boar was collected, extended, evaluated and shipped offsite. Upon arrival, the semen was reevaluated and utilized for artificial insemination of 89 commercial gilts, at least 12 gilts per boar, producing 625 piglets. Pregnancy rates were determined at day 30 and 110 of gestation; and farrowing rate and gestation length were recorded. Differences were observed in some of the semen characteristics analyzed with the clones usually possessing superior semen quality to the control, this likely being a result of age differences amongst the clones and controls. Additionally no differences were noted between the clones and controls (progenitor) or between individual boars within genetic line for pregnancy rates, gestation length or any of the litter parameters examined between the clones and controls. These data further support previous reports with limited numbers that the reproductive capabilities of cloned boars are equal to that of conventionally produced boars.
本研究比较了通过体细胞核移植产生的公猪与传统育种产生的公猪的繁殖性能。选择了两种不同的基因型进行比较:终端杂交系1(TX1)和终端杂交系2(TX2)。从TX1中选择用于比较的公猪是三头克隆公猪,由体细胞核移植产生以及克隆猪的传统生产的亲本。从TX2中选择用于比较的公猪是一头通过体细胞核移植产生的克隆公猪和两头传统生产的半同胞公猪,它们是克隆猪亲本的后代。收集每头公猪的精液,进行稀释、评估并运往外地。到达后,对精液进行重新评估,并用于对89头商业后备母猪进行人工授精,每头公猪至少授精12头后备母猪,共产下625头仔猪。在妊娠第30天和第110天测定妊娠率;记录产仔率和妊娠期长度。在分析的一些精液特征中观察到差异,克隆猪的精液质量通常优于对照猪,这可能是克隆猪和对照猪年龄差异的结果。此外,在克隆猪和对照猪(亲本)之间或同一遗传系内的个体公猪之间,在妊娠率、妊娠期长度或克隆猪和对照猪之间检查的任何窝产参数方面均未发现差异。这些数据进一步支持了之前数量有限的报告,即克隆公猪的繁殖能力与传统生产的公猪相当。