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印度种公牛的克隆:举措与挑战。

Cloning of breeding buffalo bulls in India: Initiatives & challenges.

机构信息

Division of Animal Physiology & Reproduction, ICAR-Central Institute for Research on Buffaloes, Hisar, India.

出版信息

Indian J Med Res. 2018 Dec;148(Suppl):S120-S124. doi: 10.4103/ijmr.IJMR_2103_17.

DOI:10.4103/ijmr.IJMR_2103_17
PMID:30964089
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6469371/
Abstract

The term animal cloning refers to an asexual mean of reproduction to produce genetically identical copies of any animal without the use of sperm. In India, the cloning of buffalo is well established and clones of the Murrah, the best dairy breed of buffalo, have been produced. The most acclaimed example is the restoration of progeny-tested breeding bull by isolating somatic cells from frozen doses of semen, which were stored for more than a decade in the semen bank. Buffalo bull cloning is considered the best available option to reproduce declared proven bulls and their semen would contribute to accomplishing the demand of ever-growing frozen semen, which is the prime requirement of conventional breeding. This article highlights the importance of buffalo bull cloning and its current status in India.

摘要

术语“动物克隆”是指一种无性繁殖方式,用于在不使用精子的情况下产生任何动物的遗传完全相同的复制品。在印度,水牛的克隆已经得到很好的确立,并且已经生产出了穆拉雅(Murrah)的克隆,这是最好的奶牛品种的水牛。最引人注目的例子是通过从冷冻精液剂量中分离出体细胞来恢复经过后代测试的种公牛,这些精液在精液库中已经储存了十多年。水牛公牛克隆被认为是繁殖已宣布的优良种公牛的最佳选择,它们的精液将有助于满足不断增长的冷冻精液的需求,这是常规繁殖的主要要求。本文强调了水牛公牛克隆的重要性及其在印度的现状。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/029e/6469371/7ec1a651cbb9/IJMR-148-120-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/029e/6469371/7ec1a651cbb9/IJMR-148-120-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/029e/6469371/7ec1a651cbb9/IJMR-148-120-g001.jpg

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Cloning of breeding buffalo bulls in India: Initiatives & challenges.印度种公牛的克隆:举措与挑战。
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2
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本文引用的文献

1
An update: Reproductive handmade cloning of water buffalo (Bubalus bubalis).最新进展:水牛(Bubalus bubalis)的生殖性手工克隆
Anim Reprod Sci. 2018 Oct;197:1-9. doi: 10.1016/j.anireprosci.2018.08.003. Epub 2018 Aug 9.
2
Cloning of Buffalo, a Highly Valued Livestock Species of South and Southeast Asia: Any Achievements?克隆水牛:南亚和东南亚高价值家畜物种的克隆有哪些成果?
Cell Reprogram. 2018 Apr;20(2):89-98. doi: 10.1089/cell.2017.0051. Epub 2018 Mar 16.
3
Treatment of Donor Cells and Reconstructed Embryos with a Combination of Trichostatin-A and 5-aza-2'-Deoxycytidine Improves the Developmental Competence and Quality of Buffalo Embryos Produced by Handmade Cloning and Alters Their Epigenetic Status and Gene Expression.
用曲古抑菌素A和5-氮杂-2'-脱氧胞苷联合处理供体细胞和重构胚胎可提高手工克隆水牛胚胎的发育能力和质量,并改变其表观遗传状态和基因表达。
Cell Reprogram. 2017 Jun;19(3):208-215. doi: 10.1089/cell.2016.0061. Epub 2017 May 2.
4
Valproic Acid Increases Histone Acetylation and Alters Gene Expression in the Donor Cells But Does Not Improve the In Vitro Developmental Competence of Buffalo (Bubalus bubalis) Embryos Produced by Hand-Made Cloning.丙戊酸可增加供体细胞中的组蛋白乙酰化并改变基因表达,但不能提高手工克隆水牛胚胎的体外发育能力。
Cell Reprogram. 2017 Feb;19(1):10-18. doi: 10.1089/cell.2016.0029. Epub 2017 Jan 5.
5
Changes in genetic selection differentials and generation intervals in US Holstein dairy cattle as a result of genomic selection.基因组选择对美国荷斯坦奶牛遗传选择差异和世代间隔的影响。
Proc Natl Acad Sci U S A. 2016 Jul 12;113(28):E3995-4004. doi: 10.1073/pnas.1519061113. Epub 2016 Jun 27.
6
Artificial cloning of domestic animals.家畜的人工克隆
Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):8874-8. doi: 10.1073/pnas.1501718112.
7
Buffalo embryos produced by handmade cloning from oocytes selected using brilliant cresyl blue staining have better developmental competence and quality and are closer to embryos produced by in vitro fertilization in terms of their epigenetic status and gene expression pattern.通过手工克隆从使用灿烂甲酚蓝染色选择的卵母细胞产生的水牛胚胎具有更好的发育能力和质量,并且在表观遗传状态和基因表达模式方面更接近体外受精产生的胚胎。
Cell Reprogram. 2015 Apr;17(2):141-50. doi: 10.1089/cell.2014.0077.
8
Treatment of buffalo (Bubalus bubalis) donor cells with trichostatin A and 5-aza-2'-deoxycytidine alters their growth characteristics, gene expression and epigenetic status and improves the in vitro developmental competence, quality and epigenetic status of cloned embryos.用曲古抑菌素A和5-氮杂-2'-脱氧胞苷处理水牛(Bubalus bubalis)供体细胞,可改变其生长特性、基因表达和表观遗传状态,并提高克隆胚胎的体外发育能力、质量和表观遗传状态。
Reprod Fertil Dev. 2016 Apr;28(6):824-37. doi: 10.1071/RD14176.
9
Hope for restoration of dead valuable bulls through cloning using donor somatic cells isolated from cryopreserved semen.希望通过使用从冷冻保存精液中分离出的供体体细胞进行克隆来恢复死去的珍贵公牛。
PLoS One. 2014 Mar 10;9(3):e90755. doi: 10.1371/journal.pone.0090755. eCollection 2014.
10
The endometrium responds differently to cloned versus fertilized embryos.子宫内膜对克隆胚胎和受精胚胎的反应不同。
Proc Natl Acad Sci U S A. 2009 Apr 7;106(14):5681-6. doi: 10.1073/pnas.0811841106. Epub 2009 Mar 23.