Daruwalla Jurstine, Christophi Chris
University of Melbourne, Department of Surgery Austin Hospital, Level 8 Lance Townsend Bldg., Studley Rd, Heidelberg, Vic., 3084, Australia.
Eur J Cancer. 2006 Dec;42(18):3304-11. doi: 10.1016/j.ejca.2006.08.004. Epub 2006 Sep 28.
Hyperbaric oxygen (HBO) therapy involves the administration of 100% oxygen at high pressure. It has been used to treat a variety of conditions including non-healing wounds, carbon monoxide poisoning, and as an adjuvant to radiotherapy or chemotherapy. The effect of HBO alone on the growth of malignancy remains controversial. This study investigates the impact of HBO on tumour growth, kinetics and microcirculation of colorectal cancer liver metastases in an experimental model.
Male CBA mice were induced with colorectal liver metastases via an intrasplenic injection of a murine derived colorectal cell line. Tumours were examined using quantitative stereological analysis, histology and scanning electron microscopy of microvascular resin casts. The effect of HBO on tumour proliferation and apoptosis was quantified using immunohistochemistry.
Daily exposure to HBO at 2.4 atm for 90 min had no effect on the volume of liver metastases. At day 13, HBO caused a significant reduction in tumour necrosis and proliferation compared to the non-HBO group (p=0.002 and p=0.008, respectively). By day 25 however, no differences were observed (p>0.05). No differences in apoptosis or microvascular architecture were observed.
HBO did not have a tumour stimulatory effect on colorectal liver metastases and may potentially be used safely in conjunction with other therapeutic treatment modalities.
高压氧（HBO）治疗是在高压下给予100%的氧气。它已被用于治疗多种病症，包括难愈合伤口、一氧化碳中毒，以及作为放疗或化疗的辅助手段。单独使用HBO对恶性肿瘤生长的影响仍存在争议。本研究在一个实验模型中探究了HBO对结直肠癌肝转移瘤生长、动力学及微循环的影响。
通过脾内注射鼠源性结直肠癌细胞系诱导雄性CBA小鼠发生结直肠癌肝转移。使用定量体视学分析、组织学及微血管树脂铸型扫描电子显微镜对肿瘤进行检查。采用免疫组织化学法定量分析HBO对肿瘤增殖和凋亡的影响。
每天在2.4个大气压下暴露于HBO 90分钟对肝转移瘤体积没有影响。在第13天，与未接受HBO治疗的组相比，HBO使肿瘤坏死和增殖显著减少（分别为p = 0.002和p = 0.008）。然而，到第25天，未观察到差异（p>0.05）。未观察到凋亡或微血管结构方面的差异。
HBO对结直肠癌肝转移瘤没有促肿瘤作用，可能可以安全地与其他治疗方式联合使用。
