Ghaedi Mehrorang, Ahmadi Farshid, Shokrollahi Ardeshir
Chemistry Department, Yasouj University, Yasouj 75914-353, Iran.
J Hazard Mater. 2007 Apr 2;142(1-2):272-8. doi: 10.1016/j.jhazmat.2006.08.012. Epub 2006 Aug 12.
A sensitive and simple method for the simultaneous preconcentration of nutritionally important minerals in real samples has been reported. The method is based on the formation of metal complexes by 4,6-dihydroxy-2-mercaptopyrimidine (DHMP) loaded on activated carbon. The metals content on the complexes are then eluted using 5 mL 2M HNO(3) in acetone, which are detected by AAS at resonance line. In this procedure, minerals such as Cu, Ni, Pb and Co could be analyzed in one run by caring out the simultaneous separation and quantification of them. At optimum condition the response are linear over concentration range of 0.04-1.1 microg mL(-1) for Ni(2+) and 0.04-1.0 microg mL(-1) for Cu(2+), Pb(2+) and Co(2+). The detection limits of each element are expressed as the amount of analytes in ng mL(-1) giving a signal to noise ratio of 3 are equal to 3.5, 3.4, 2.9 and 8.4 for Ni(2+), Co(2+), Cu(2+) and Pb(2+). The sorption capacity was determined by saturating 0.5 g solid phase. The loading capacity are 0.54, 0.53, 0.63 and 0.45 mg g(-1) for Ni(2+), Co(2+), Cu(2+) and Pb(2+). The ability of method for repeatable recovery of trace ion are 99.0, 98.9, 99.2 and 98.8 with R.S.D. of 1.4, 1.3, 1.2 and 1.4 for Ni(2+), Co(2+), Cu(2+) and Pb(2+). The low detection limits of these elements in this technique make it a superior alternative to UV-vis and in several applications, also an alternative to ICP-MS techniques. The method has been successfully applied for these metals content evaluation in some real samples including natural water, leaves of spinach and cow liver.
已报道了一种用于同时预富集实际样品中具有营养重要性的矿物质的灵敏且简便的方法。该方法基于负载在活性炭上的4,6 - 二羟基 - 2 - 巯基嘧啶（DHMP）形成金属配合物。然后使用5 mL 2M硝酸（HNO₃）的丙酮溶液洗脱配合物上的金属含量，通过原子吸收光谱法（AAS）在共振线处进行检测。在此过程中，通过同时分离和定量分析，一次运行即可分析诸如铜（Cu）、镍（Ni）、铅（Pb）和钴（Co）等矿物质。在最佳条件下，对于镍离子（Ni²⁺），响应在0.04 - 1.1 μg mL⁻¹的浓度范围内呈线性；对于铜离子（Cu²⁺）、铅离子（Pb²⁺）和钴离子（Co²⁺），响应在0.04 - 1.0 μg mL⁻¹的浓度范围内呈线性。各元素的检测限以给出3倍信噪比的纳克每毫升（ng mL⁻¹）分析物量表示，镍离子（Ni²⁺）、钴离子（Co²⁺）、铜离子（Cu²⁺）和铅离子（Pb²⁺）分别等于3.5、3.4、2.9和8.4。通过使0.5 g固相饱和来测定吸附容量。镍离子（Ni²⁺）、钴离子（Co²⁺）、铜离子（Cu²⁺）和铅离子（Pb²⁺）的负载容量分别为0.54、0.53、0.63和0.45 mg g⁻¹。该方法对痕量离子的可重复回收率分别为镍离子（Ni²⁺）99.0、钴离子（Co²⁺）98.9、铜离子（Cu²⁺）99.2和铅离子（Pb²⁺）98.8，相对标准偏差（R.S.D.）分别为1.4、1.3、1.2和1.4。该技术中这些元素的低检测限使其成为紫外可见光谱法的优越替代方法，在一些应用中，也是电感耦合等离子体质谱法（ICP - MS）技术的替代方法。该方法已成功应用于一些实际样品中这些金属含量的评估，包括天然水、菠菜叶和牛肝。
