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通过液相色谱/紫外/质谱分析鉴定冬青叶美登木和阔叶美登木叶中的黄酮醇(卫矛科)。

Identification of flavonols in leaves of Maytenus ilicifolia and M. aquifolium (Celastraceae) by LC/UV/MS analysis.

作者信息

Tiberti Luciana A, Yariwake Janete H, Ndjoko Karine, Hostettmann Kurt

机构信息

Universidade de São Paulo, Instituto de Química de São Carlos, Caixa Postal 780, 13560-970 São Carlos, SP, Brazil.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Feb 1;846(1-2):378-84. doi: 10.1016/j.jchromb.2006.09.014. Epub 2006 Oct 2.

Abstract

A comparative analysis of the flavonoid components of the leaves of two medicinal plants known in Brazil as "espinheira santa", namely, Maytenus ilicifolia Mart. ex Reiss. and M. aquifolium Mart. (Celastraceae), and a hybrid plant, M. aquifoliumxM. ilicifolia, has been carried out using high-performance liquid chromatography coupled with photodiode array UV detection and mass spectrometry. One methoxyflavonoid glycoside and 18 flavonol-3-O-glycosides were identified in the extracts on the basis of their on-line UV spectra (measured in the absence and presence of shift reagents) and multiple stage mass spectral data. Fingerprint analysis of the flavonoid extracts revealed significant differences in the profiles of the two Maytenus species, while the hybrid plant contained flavonoids found in both parent species.

摘要

对巴西两种被称为“espinheira santa”的药用植物——即冬青叶美登木(Maytenus ilicifolia Mart. ex Reiss.)和阔叶美登木(M. aquifolium Mart.)(卫矛科)以及一种杂交植物阔叶美登木×冬青叶美登木的叶子中的黄酮类成分进行了比较分析,采用了高效液相色谱结合光电二极管阵列紫外检测和质谱法。根据提取物的在线紫外光谱(在不存在和存在位移试剂的情况下测量)和多级质谱数据,鉴定出了一种甲氧基黄酮苷和18种黄酮醇-3-O-糖苷。黄酮类提取物的指纹图谱分析显示,两种美登木属植物的图谱存在显著差异,而杂交植物含有在两个亲本物种中都能找到的黄酮类化合物。

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