Lhuillier Amélie, Fabre Nicolas, Moyano Florian, Martins Nathalie, Claparols Catherine, Fourasté Isabelle, Moulis Claude
UMR-152 IRD-UPS Laboratoire Pharmacochimie des Substances Naturelles et Pharmacophores Redox, Faculté des Sciences Pharmaceutiques, Université Paul-Sabatier, F-31062 Toulouse cedex 09, France.
J Chromatogr A. 2007 Aug 10;1160(1-2):13-20. doi: 10.1016/j.chroma.2007.03.038. Epub 2007 Mar 16.
Liquid chromatography (LC) coupled to negative electrospray ionisation (ESI) tandem mass spectrometry (MS/MS) was used for the rapid and sensitive identification of flavonoid compounds in Agauria salicifolia. The leaf flavonoid content in individual of A. salicifolia originating from population with contrasted ecogeographical situation and morphological characteristics was found to be variable qualitatively and highly variable quantitatively. Identification of the compounds was carried out by interpretation of UV, MS and MS/MS spectra. Fourteen flavonoids were identified, all of which had not previously been reported in Agauria spp. Two flavonol-O-glucuronides were found to differentiate the two populations.
液相色谱（LC）与负电喷雾电离（ESI）串联质谱（MS/MS）联用，用于快速、灵敏地鉴定柳叶阿加里亚中的黄酮类化合物。研究发现，来自具有不同生态地理状况和形态特征种群的柳叶阿加里亚个体中的叶片黄酮含量在质量上存在差异，在数量上变化很大。通过对紫外光谱、质谱和串联质谱的解析来鉴定化合物。共鉴定出14种黄酮类化合物，所有这些化合物此前均未在阿加里亚属植物中报道过。发现两种黄酮醇-O-葡萄糖醛酸苷可区分这两个种群。
