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通过冷冻电子显微镜观察到的DNA小环形状的三维重建与比较

3D reconstruction and comparison of shapes of DNA minicircles observed by cryo-electron microscopy.

作者信息

Amzallag Arnaud, Vaillant Cédric, Jacob Mathews, Unser Michael, Bednar Jan, Kahn Jason D, Dubochet Jacques, Stasiak Andrzej, Maddocks John H

机构信息

Laboratory for Computation and Visualization in Mathematics and Mechanics, EPFL FSB IMB, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

出版信息

Nucleic Acids Res. 2006;34(18):e125. doi: 10.1093/nar/gkl675. Epub 2006 Sep 29.

DOI:10.1093/nar/gkl675
PMID:17012274
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1635295/
Abstract

We use cryo-electron microscopy to compare 3D shapes of 158 bp long DNA minicircles that differ only in the sequence within an 18 bp block containing either a TATA box or a catabolite activator protein binding site. We present a sorting algorithm that correlates the reconstructed shapes and groups them into distinct categories. We conclude that the presence of the TATA box sequence, which is believed to be easily bent, does not significantly affect the observed shapes.

摘要

我们使用冷冻电子显微镜来比较158个碱基对长的DNA小环的三维形状，这些小环仅在一个18个碱基对的区域内的序列不同，该区域包含一个TATA盒或一个分解代谢物激活蛋白结合位点。我们提出了一种排序算法，该算法将重建的形状相关联并将它们分组到不同的类别中。我们得出结论，据信容易弯曲的TATA盒序列的存在不会显著影响观察到的形状。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caa1/1636443/6165924ec6cd/gkl675f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caa1/1636443/4660e0d077cc/gkl675f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caa1/1636443/11c0b079036e/gkl675f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caa1/1636443/cc8c0c9c941b/gkl675f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caa1/1636443/1ea15f7bdd63/gkl675f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caa1/1636443/d99a6f9edbe0/gkl675f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caa1/1636443/6377262f0f7c/gkl675f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caa1/1636443/234c8397d22b/gkl675f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caa1/1636443/6165924ec6cd/gkl675f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caa1/1636443/4660e0d077cc/gkl675f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caa1/1636443/11c0b079036e/gkl675f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caa1/1636443/cc8c0c9c941b/gkl675f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caa1/1636443/1ea15f7bdd63/gkl675f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caa1/1636443/d99a6f9edbe0/gkl675f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caa1/1636443/6377262f0f7c/gkl675f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caa1/1636443/234c8397d22b/gkl675f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caa1/1636443/6165924ec6cd/gkl675f8.jpg

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