Mizrokhi L J, Mazo A M
Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Proc Natl Acad Sci U S A. 1990 Dec;87(23):9216-20. doi: 10.1073/pnas.87.23.9216.
We addressed the possibility of the horizontal transfer of long interspersed element (LINE)-like mobile elements by studying the distribution of the Drosophila melanogaster LINE-like element jockey in different Drosophila species. Outside the D. melanogaster group jockey was detected only in the distantly related species Drosophila funebris. Cloning and sequencing of this element from D. funebris revealed the existence of the two open reading frames highly similar to those of jockey from D. melanogaster. Elements from both species are transcriptionally active and contain in their promoter regions a conserved sequence important for its activity. The high degree of similarity between the D. melanogaster and the D. funebris jockey and the absence of jockey from other sibling species of the D. funebris group provide evidence for the horizontal transmission of jockey into D. funebris.
我们通过研究黑腹果蝇中类长散在重复元件（LINE）的移动元件——骑师元件（jockey）在不同果蝇物种中的分布，探讨了骑师元件水平转移的可能性。在黑腹果蝇组之外，仅在亲缘关系较远的物种果蝇（Drosophila funebris）中检测到骑师元件。从果蝇（D. funebris）中克隆并测序该元件，发现存在两个与黑腹果蝇的骑师元件高度相似的开放阅读框。两个物种的元件均具有转录活性，且其启动子区域含有对其活性至关重要的保守序列。黑腹果蝇和果蝇（D. funebris）的骑师元件之间的高度相似性，以及果蝇（D. funebris）组其他姐妹物种中不存在骑师元件，为骑师元件向果蝇（D. funebris）的水平转移提供了证据。
