Ohtsuka Jun, Nagata Koji, Lee Woo Cheol, Ono Yusuke, Fukuda Ryouichi, Ohta Akinori, Tanokura Masaru
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Oct 1;62(Pt 10):1003-5. doi: 10.1107/S1744309106035561. Epub 2006 Sep 30.
phosphoethanolamine cytidylyltransferase (ECT) is the enzyme that catalyzes the conversion of phosphoethanolamine to CDP-ethanolamine in the phosphatidylethanolamine-biosynthetic pathway (Kennedy pathway). ECT from Saccharomyces cerevisiae was crystallized by the sitting-drop vapour-diffusion method using PEG 4000 as precipitant. The crystals diffracted X-rays from a synchrotron-radiation source to 1.88 A resolution. The space group was assigned as primitive tetragonal, P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 66.3, c = 150.8 A. The crystals contain one ECT molecule in the asymmetric unit (V(M) = 2.2 A(3) Da(-1)), with a solvent content of 43%.
磷酸乙醇胺胞苷酰转移酶（ECT）是在磷脂酰乙醇胺生物合成途径（肯尼迪途径）中催化磷酸乙醇胺转化为CDP - 乙醇胺的酶。使用聚乙二醇4000作为沉淀剂，通过坐滴气相扩散法使来自酿酒酵母的ECT结晶。晶体从同步辐射源衍射X射线至1.88 Å分辨率。空间群被指定为原始四方晶系，P4(1)2(1)2或P4(3)2(1)2，晶胞参数a = b = 66.3，c = 150.8 Å。晶体在不对称单元中包含一个ECT分子（V(M) = 2.2 ų Da⁻¹），溶剂含量为43%。
