Freeman M L, Dewey W C, Hopwood L E
J Natl Cancer Inst. 1977 Jun;58(6):1837-9. doi: 10.1093/jnci/58.6.1837.
Chinese hamster ovary cells incubated with various concentrations of CO2, to obtain extracellular pH values in the range of 6.40-7.85, were heated at 45.5C for 5, 10, or 20 minutes. Thermal sensitivity increased sharply from pH 7.35 to 6.65 (i.e., survival decreased from 1 X 10(-2) to 3 X 10(-5) for 20 minutes of heating), but remained constant from pH 7.35 to 7.85. The enhanced thermal sensitivity at pH values below pth 7.35 suggested that tumors should be preferentially destroyed by heat relative to normal tissue, since reports indicated that tumors were more acidic than the surrounding normal tissue.
将中国仓鼠卵巢细胞与不同浓度的二氧化碳孵育，以获得细胞外pH值在6.40 - 7.85范围内，然后在45.5℃下加热5、10或20分钟。热敏感性从pH 7.35到6.65急剧增加（即加热20分钟时存活率从1×10⁻²降至3×10⁻⁵），但从pH 7.35到7.85保持恒定。pH值低于7.35时增强的热敏感性表明，相对于正常组织，肿瘤应优先被热破坏，因为有报告指出肿瘤比周围正常组织酸性更强。
