Loreau N, Boiziau C, Verspieren P, Shire D, Toulmé J J
Laboratoire de Biophysique, INSERM U201, CNRS UA 481, Paris, France.
FEBS Lett. 1990 Nov 12;274(1-2):53-6. doi: 10.1016/0014-5793(90)81327-k.
Synthetic oligodeoxynucleotides, either unmodified or linked to an intercalating agent, have been used to prevent cDNA elongation by the AMV reverse transcriptase. Oligonucleotide/RNA hybrids specifically arrest primer extension. The blockage involves the degradation of the RNA part bound to the antisense oligonucleotide by the RNase-H activity associated with the retroviral polymerase.
合成的寡脱氧核苷酸，无论是未修饰的还是与嵌入剂相连的，都已被用于通过禽成髓细胞瘤病毒（AMV）逆转录酶来阻止cDNA的延伸。寡核苷酸/RNA杂交体可特异性地阻止引物延伸。这种阻断涉及与逆转录病毒聚合酶相关的RNase-H活性对与反义寡核苷酸结合的RNA部分的降解。
