Sagredo E, Pino A, Ibañez P
Departamento de Biología Celular y Ciencias Morfológicas, Facultad de Farmacia, Universidad Del Pais Vasco, Alava España.
J Biol Buccale. 1990 Sep;18(3):163-8.
The number/mm2 of sections of Langerhans cells (LC's) expressing antigens T6 and/or HLA-DR was determined in eight human gingival samples which consisting of normal gingivae, contraceptive induced hyperplastic, traumatic, inflammatory and diphenylhydantoin hyperplasic gingivae. All samples, except those from normal gingivae, showed inflammatory and/or hyperplasic histopathological patterns. In the immunostained samples, LC's appeared in variable numbers and were randomly distributed between the keratinocytes. All pathological samples, except the inflammatory gingiva showed HLA-DR+ keratinocytes. Wilcoxon and Mann-Whitney statistical tests suggested the existence of three subpopulations of LC's: T6+/DR+; T6-/DR+ and T6+/DR-. The subpopulation T6+/DR+ was predominant in conditions of normal immune response. The subpopulation T6-/DR+ suggested the presence of newly incorporated LC's from the lamina propia. T6+/DR- subpopulations could indicate the existence of a system to avoid overstimulation. It is concluded that quantitative evaluation of T6 and HLA-DR expression was representative of the gingival conditions and the disease activity.
在八个取自人类牙龈的样本中，测定了表达抗原T6和/或HLA-DR的朗格汉斯细胞（LC）每平方毫米的数量。这些样本包括正常牙龈、避孕药引起的增生性牙龈、创伤性牙龈、炎性牙龈和苯妥英钠增生性牙龈。除了正常牙龈的样本外，所有样本均呈现出炎性和/或增生性组织病理学模式。在免疫染色样本中，LC数量不等，随机分布于角质形成细胞之间。除炎性牙龈外，所有病理样本均显示HLA-DR阳性角质形成细胞。Wilcoxon和Mann-Whitney统计检验表明存在三种LC亚群：T6+/DR+；T6-/DR+和T6+/DR-。T6+/DR+亚群在正常免疫反应条件下占主导地位。T6-/DR+亚群提示存在从固有层新迁入的LC。T6+/DR-亚群可能表明存在一种避免过度刺激的机制。结论是，T6和HLA-DR表达的定量评估代表了牙龈状况和疾病活动。
