Betbeze Caroline M, Wu Ching Ching, Krohne Sheryl G, Stiles Jean
Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA.
Am J Vet Res. 2006 Oct;67(10):1788-93. doi: 10.2460/ajvr.67.10.1788.
To evaluate the in vitro antifungal properties of silver sulfadiazine (SSD) and natamycin against filamentous fungi isolated from eyes of horses with keratomycosis.
Filamentous fungal isolates obtained from eyes of keratomycosis-affected horses.
Fungal culture of ocular samples yielded 6 Fusarium spp; 7 Aspergillus spp; and 1 isolate each of Curvularia, Scopulariopsis, Penicillium, and Chrysosporium. For each fungal isolate, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of SSD and natamycin were determined.
For all 17 fungal isolates, SSD MIC distribution ranged from < or = 1 to > 64 microg/mL; MIC50 and MIC90 (MICs at which 50% and 90% of organisms were inhibited) were 4 and 32 microg/mL, respectively. The SSD MFC distribution for all isolates was < or = 1 to > 64 microg/mL; MFC50 and MFC90 (MFCs at which 50% and 90% of organisms were killed) were 8 and > 64 microg/mL, respectively. For all fungal isolates, natamycin MIC distribution ranged from 256 to > 1,000 microg/mL; MIC50 and MIC90 were 512 and > 1,000 microg/mL, respectively. The natamycin MFC distribution for all isolates ranged from 512 to > 1,000 microg/mL; MFC(50) and MFC(90) were each > 1,000 microg/mL.
These in vitro data suggest that SSD is fungicidal against the fungal isolates that were obtained from eyes of horses with keratomycosis and that natamycin is fungicidal against some of the isolates at the drug concentrations evaluated. Silver sulfadiazine may be a therapeutic option for equine keratomycosis.
评估磺胺嘧啶银（SSD）和那他霉素对从患有角膜真菌病的马眼中分离出的丝状真菌的体外抗真菌特性。
从患有角膜真菌病的马眼中获得的丝状真菌分离株。
眼部样本的真菌培养产生了6株镰刀菌属；7株曲霉菌属；以及弯孢属、帚霉属、青霉属和金孢子菌属各1株分离株。对于每种真菌分离株，测定了SSD和那他霉素的最低抑菌浓度（MIC）和最低杀菌浓度（MFC）。
对于所有17株真菌分离株，SSD的MIC分布范围为≤1至＞64μg/mL；MIC50和MIC90（抑制50%和90%生物体生长的MIC）分别为4和32μg/mL。所有分离株的SSD MFC分布为≤1至＞64μg/mL；MFC50和MFC90（杀死50%和90%生物体的MFC）分别为8和＞64μg/mL。对于所有真菌分离株，那他霉素的MIC分布范围为256至＞1000μg/mL；MIC50和MIC90分别为512和＞1000μg/mL。所有分离株的那他霉素MFC分布范围为512至＞1000μg/mL；MFC50和MFC90均＞1000μg/mL。
这些体外数据表明，SSD对从患有角膜真菌病的马眼中分离出的真菌分离株具有杀菌作用，并且在评估的药物浓度下，那他霉素对某些分离株具有杀菌作用。磺胺嘧啶银可能是马角膜真菌病的一种治疗选择。
